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PMID: 18335213 Published · ppublish English Journal Article Review

Microtechnologies for membrane protein studies.

Analytical and bioanalytical chemistry ·Vol. 391 ·No. 8 ·2008-08-00 ·Pages 2695-702

Suzuki H, Takeuchi S

Abstract

Despite the rapid and enormous progress in biotechnologies, the biochemical analysis of membrane proteins is still a difficult task. The presence of the large hydrophobic region buried in the lipid bilayer membrane (transmembrane domain) makes it difficult to analyze membrane proteins in standard assays developed for water-soluble proteins. To handle membrane proteins, the lipid bilayer membrane may be used as a platform to sustain their functionalities. Relatively slow progress in developing micro total analysis systems (microTAS) for membrane protein analysis directly reflects the difficulty of handling lipid membranes, which is a common problem in bulk measurement technologies. Nonetheless, researchers are continuing to develop efficient and sensitive analytical microsystems for the study of membrane proteins. Here, we review the latest developments, which enable detection of events caused by membrane proteins, such as ion channel current, membrane transport, and receptor/ligand interaction, by utilizing microfabricated structures. High-throughput and highly sensitive detection systems for membrane proteins are now becoming a realistic goal. Although most of these systems are still in the early stages of development, we believe this field will become one of the most important applications of microTAS for pharmaceutical and clinical screenings as well as for basic biochemical research.

MeSH Terms
Lipid Bilayers/chemistry Membrane Transport Proteins/chemistry Microchemistry/methods,trends Patch-Clamp Techniques/methods Technology, Pharmaceutical/methods,trends
Chemicals
Lipid Bilayers Membrane Transport Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Suzuki Hiroaki
Institute of Industrial Science, The University of Tokyo, Komaba 4-6-1, Meguro-ku, Tokyo, 153-8505, Japan. suzuki@ist.osaka-u.ac.jp
Takeuchi Shoji
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Article Info
Journal
Analytical and bioanalytical chemistry
Abbr.
Anal Bioanal Chem
ISSN
1618-2650
DOI
10.1007/s00216-008-1916-0
Email
suzuki@ist.osaka-u.ac.jp
Published
2008-08-00
Epub
2008-00-12
Pages
2695-702
Language
English
Region
Germany
NLM ID
101134327
PMCID
PMC2516541
Subset
IM
External Links
PubMed source DOI: 10.1007/s00216-008-1916-0 PMC Full Text View journal details
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