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PMID: 15356342 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Visualization of plasma membrane compartmentalization with patterned lipid bilayers.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 38 ·2004-09-21 ·Pages 13798-803

Wu M, Holowka D, Craighead HG, Baird B

Abstract

Micrometer-size patterned lipid bilayers containing liganded lipids are used to control the location and size of receptor clusters and enable direct visualization of structural reorganization of cellular components. Subsequent to concentration of Fcepsilon receptor I, the mast cell receptor for IgE, and colocalized tyrosine phosphorylation activity, Lyn kinase and other proteins anchored to the inner leaflet of the plasma membrane redistribute selectively with the receptor clusters in a process that depends on actin polymerization. Surprisingly, outer leaflet components characteristically associated with lipid rafts do not detectably coredistribute with these inner leaflet components. Cell activation using patterned surfaces provides unique insights into cell membrane structural organization, revealing dynamic, large-scale uncoupling of inner and outer leaflet components of lipid rafts.

MeSH Terms
Actins/chemistry Animals Cell Line, Tumor Cell Membrane Structures/ultrastructure Cytoskeleton/ultrastructure Genes, Reporter Lipid Bilayers/chemistry Membrane Lipids/chemistry Microscopy, Confocal Phosphorylation Phosphotyrosine/metabolism Protein-Tyrosine Kinases/metabolism Rats Receptors, IgE/analysis src-Family Kinases/metabolism
Chemicals
Actins Lipid Bilayers Membrane Lipids Receptors, IgE Phosphotyrosine Protein-Tyrosine Kinases lyn protein-tyrosine kinase src-Family Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wu Min
Department of Chemistry and Chemical Biology and School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.
Holowka David
Craighead Harold G
Baird Barbara
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0403835101
Published
2004-09-21
Epub
2004-00-08
Pages
13798-803
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC518836
Subset
IM
Grants
NIAID NIH HHS · R01 AI018306 · United States
NIAID NIH HHS · AI18306 · United States
External Links
PubMed source DOI: 10.1073/pnas.0403835101 PMC Full Text View journal details
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