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PMID: 1820216 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Repositioning of an alternative exon sequence of mouse IgM pre-mRNA activates splicing of the preceding intron.

Gene expression ·Vol. 1 ·No. 3 ·1991-00-00 ·Pages 175-84

Watakabe A, Sakamoto H, Shimura Y

Abstract

Using a transient expression system of mouse IgM mini-gene constructs in mouse B-cell lines and in fibroblast L cell, we investigated splicing of the IgM transcript. We observed that the efficiency of splicing between exons C4 and M1 (C4-to-M1 splicing), the splicing reaction leading to the production of membrane-bound form (microns) mRNA, was drastically affected by mutations in a specific portion of the downstream exon (M2). The results show that the specific exon M2 sequence activates the C4-to-M1 splicing. This activation was not observed when splicing between exons M1 and M2 was abolished by base substitutions at the splice sites. These results indicate that positioning of the downstream exon is crucial for efficient splicing of the preceding intron.

MeSH Terms
Animals Base Sequence Exons/physiology Immunoglobulin M/genetics Introns/physiology Mice Molecular Sequence Data Mutation RNA Splicing/genetics Tumor Cells, Cultured
Chemicals
Immunoglobulin M
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Watakabe A
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Sakamoto H
Shimura Y
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45 references, click to expand
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Article Info
Journal
Gene expression
Abbr.
Gene Expr
ISSN
1052-2166
Published
1991-00-00
Pages
175-84
Language
English
Region
United States
NLM ID
9200651
PMCID
PMC5952188
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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