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PMID: 3352607 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The length of the downstream exon and the substitution of specific sequences affect pre-mRNA splicing in vitro.

Molecular and cellular biology ·Vol. 8 ·No. 2 ·1988-02-00 ·Pages 860-6

Furdon PJ, Kole R

Abstract

We have shown previously that truncation of the human beta-globin pre-mRNA in the second exon, 14 nucleotides downstream from the 3' splice site, leads to inhibition of splicing but not cleavage at the 5' splice site. We now show that several nonglobin sequences substituted at this site can restore splicing and that the efficiency of splicing depends on the length of the second (downstream) exon and not a specific sequence. Deletions in the first exon have no effect on the efficiency of in vitro splicing. Surprisingly, an intron fragment from the 5' region of the human or rabbit beta-globin intron 2, when placed 14 nucleotides downstream from the 3' splice site, inhibited all the steps in splicing beginning with cleavage at the 5' splice site. This result suggests that the intron 2 fragment carries a "poison" sequence that can inhibit the splicing of an upstream intron.

MeSH Terms
Base Sequence Exons Genes Globins/genetics Humans Introns Molecular Sequence Data Plasmids RNA Precursors/genetics RNA Splicing Transcription, Genetic
Chemicals
RNA Precursors Globins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Furdon P J
Lineberger Cancer Research Center, University of North Carolina, Chapel Hill 27514.
Kole R
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31 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.2.860-866.1988
Published
1988-02-00
Pages
860-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363217
Subset
IM
Grants
NIGMS NIH HHS · GM32994 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.2.860-866.1988 PMC Full Text View journal details
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