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PMID: 2510128 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A secondary structure at the 3' splice site affects the in vitro splicing reaction of mouse immunoglobulin mu chain pre-mRNAs.

Nucleic acids research ·Vol. 17 ·No. 20 ·1989-10-25 ·Pages 8159-69

Watakabe A, Inoue K, Sakamoto H, Shimura Y

Abstract

The expression of the IgM (immunoglobulin mu) heavy chain gene is known to be regulated at the post-transcriptional level. The two isoforms, the membrane-bound and secreted forms, are generated from the same gene by alternative processing at the 3' end of the primary transcript. The processing reactions involved are polyadenylation at the upstream poly(A) site (for the secreted form) and polyadenylation at the downstream poly(A) site coupled with splicing between exon C4 and exon M1 (for the membrane-bound form). The regulatory mechanism underlying these differential processing reactions is still not well understood. We investigated the splicing reaction between exon C4 and exon M1 in a HeLa nuclear extract using model transcripts containing the 5' and 3' splice sites of the C4-M1 intron. We found that the 3' splice site of the C4-M1 intron is sequestered in a stem-loop structure, which inhibits the splicing reaction in vitro. The inhibition by the stem-loop structure was also observed with a mouse lymphoma extract.

MeSH Terms
Animals Base Sequence Cell Nucleus/metabolism Gene Expression Regulation Genes, Immunoglobulin HeLa Cells/immunology Humans Immunoglobulin Heavy Chains/genetics Immunoglobulin mu-Chains/genetics Mice Molecular Sequence Data Nucleic Acid Conformation Oligonucleotide Probes RNA Precursors/genetics RNA Processing, Post-Transcriptional RNA Splicing Restriction Mapping
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin mu-Chains Oligonucleotide Probes RNA Precursors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Watakabe A
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Inoue K
Sakamoto H
Shimura Y
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29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.20.8159
Published
1989-10-25
Pages
8159-69
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334955
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.20.8159 PMC Full Text View journal details
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