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PMID: 181748 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Effect of Fv-1 gene product on proviral DNA formation and integration in cells infected with murine leukemia viruses.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 7 ·1976-07-00 ·Pages 2236-40

Jolicoeur P, Baltimore D

Abstract

The amounts of unintegrated murine leukemia virus-specific DNA detected by molecular hybridization in extracts of Fv-ln/n (strains NIH/3T3, SIM) or Fv-lb/b (strains JLS-V9, SIM.R) mouse cells after infection with N- or B-tropic viruses were found to be the same in both permissive and resistant cells. Therefore, formation of DNA products from the viral RNA template does not appear to be grossly affected by the Fv-l gene product. Integration of virus-specific DNA into chromosomal cellular DNA was assayed by hybridization of radioactive complementary DNA to DNA from infected cells. With either NIH/3T3 or SIM.R cells infected with N- or B-tropic viruses, integration of proviral DNA could be detected in permissive cells but not in nonpermissive cells. The Fv-l gene product therefore appears to prevent integration of proviral DNA.

MeSH Terms
Animals DNA/metabolism DNA, Viral/biosynthesis,metabolism Genes Leukemia Virus, Murine/growth & development Mice Virus Replication
Chemicals
DNA, Viral DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jolicoeur P
Baltimore D
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.7.2236
Published
1976-07-00
Pages
2236-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430510
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.7.2236 PMC Full Text View journal details
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