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PMID: 1251192 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Proviral DNA of Moloney leukemia virus: purification and visualization.

Science (New York, N.Y.) ·Vol. 191 ·No. 4227 ·1976-02-13 ·Pages 569-71

Gianni AM, Hutton JR, Smotkin D, Weinberg RA

Abstract

Closed-circular proviral DNA of Moloney leukemia virus has been purified from a 10(7) excess of cellular and mitochondrial DNA. The DNA can be visualized in the electron microscope and has the contour length of a molecule with a moleculecular weight of about 5.5 + 10(6). Electron microscopic observation of a hybrid between viral RNA and this circular DNA confirms the viral origin of this molecule.

MeSH Terms

DNA, Circular/isolation & purification DNA, Viral/isolation & purification Microscopy, Electron Moloney murine leukemia virus/analysis,growth & development Nucleic Acid Hybridization RNA, Viral Ribonucleases

Chemicals

DNA, Circular DNA, Viral RNA, Viral Ribonucleases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gianni A M

Hutton J R

Smotkin D

Weinberg R A

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.1251192

Published

1976-02-13

Pages

569-71

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.1251192 View journal details

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