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PMID: 18162593 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis.

Plant physiology ·Vol. 146 ·No. 2 ·2008-02-00 ·Pages 623-35

Jung C, Seo JS, Han SW, Koo YJ, Kim CH, Song SI, Nahm BH, Choi YD, Cheong JJ

Abstract

AtMYB44 belongs to the R2R3 MYB subgroup 22 transcription factor family in Arabidopsis (Arabidopsis thaliana). Treatment with abscisic acid (ABA) induced AtMYB44 transcript accumulation within 30 min. The gene was also activated under various abiotic stresses, such as dehydration, low temperature, and salinity. In transgenic Arabidopsis carrying an AtMYB44 promoter-driven beta-glucuronidase (GUS) construct, strong GUS activity was observed in the vasculature and leaf epidermal guard cells. Transgenic Arabidopsis overexpressing AtMYB44 is more sensitive to ABA and has a more rapid ABA-induced stomatal closure response than wild-type and atmyb44 knockout plants. Transgenic plants exhibited a reduced rate of water loss, as measured by the fresh-weight loss of detached shoots, and remarkably enhanced tolerance to drought and salt stress compared to wild-type plants. Microarray analysis and northern blots revealed that salt-induced activation of the genes that encode a group of serine/threonine protein phosphatases 2C (PP2Cs), such as ABI1, ABI2, AtPP2CA, HAB1, and HAB2, was diminished in transgenic plants overexpressing AtMYB44. By contrast, the atmyb44 knockout mutant line exhibited enhanced salt-induced expression of PP2C-encoding genes and reduced drought/salt stress tolerance compared to wild-type plants. Therefore, enhanced abiotic stress tolerance of transgenic Arabidopsis overexpressing AtMYB44 was conferred by reduced expression of genes encoding PP2Cs, which have been described as negative regulators of ABA signaling.

MeSH Terms
Arabidopsis/drug effects,genetics,metabolism Arabidopsis Proteins/genetics,metabolism Gene Expression Profiling Gene Expression Regulation, Plant/physiology Plant Stomata/metabolism Plants, Genetically Modified Sodium Chloride/pharmacology Transcription Factors/genetics,metabolism
Chemicals
Arabidopsis Proteins MYB44 protein, Arabidopsis Transcription Factors Sodium Chloride
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Jung Choonkyun
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea.
Seo Jun Sung
Han Sang Won
Koo Yeon Jong
Kim Chung Ho
Song Sang Ik
Nahm Baek Hie
Choi Yang Do
Cheong Jong-Joo
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.107.110981
Published
2008-02-00
Epub
2007-00-27
Pages
623-35
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC2245844
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.107.110981 PMC Full Text View journal details
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