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PMID: 14767767 Published · ppublish English Journal Article

Seed-specific transcription factors ABI3 and FUS3: molecular interaction with DNA.

Planta ·Vol. 219 ·No. 1 ·2004-05-00 ·Pages 158-66

Mönke G, Altschmied L, Tewes A, Reidt W, Mock HP, Bäumlein H, Conrad U

Abstract

In Arabidopsis thaliana (L.) Heynh. the seed-specific transcription factors ABI3 and FUS3 have key regulatory functions during the development of mature seeds. The highly conserved RY motif [DNA motif CATGCA(TG)], present in many seed-specific promoters, is an essential target of both regulators. Here we show that, in vitro, the full-length ABI3 protein, as well as FUS3 protein, is able to bind to RY-DNA and that the B3 domains of both transcription factors are necessary and sufficient for the specific interaction with the RY element. Flanking sequences of the RY motif modulate the binding, but the presence of an RY sequence alone allows the specific interaction of ABI3 and FUS3 with the target in vitro. Transcriptional activity of ABI3 and FUS3, measured by transient promoter activation, requires the B3 DNA-binding domain and an activation domain. In addition to the known N-terminal-located activation domain, a second transcription activation domain was found in the B1 region of ABI3.

MeSH Terms
Arabidopsis/genetics,metabolism Arabidopsis Proteins/metabolism Base Sequence Conserved Sequence DNA, Plant/genetics,metabolism Molecular Sequence Data Oligonucleotides Promoter Regions, Genetic Protein Binding Recombinant Proteins/genetics,metabolism Seeds/genetics,metabolism Transcription Factors Transcription, Genetic Transcriptional Activation
Chemicals
ABI3 protein, Arabidopsis Arabidopsis Proteins DNA, Plant Oligonucleotides Recombinant Proteins Transcription Factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Mönke Gudrun
Institut für Pflanzengenetik und Kulturpflanzenforschung Gatersleben, Corrensstr.3, 6466 Gatersleben, Germany.
Altschmied Lothar
Tewes Annegret
Reidt Wim
Mock Hans-Peter
Bäumlein Helmut
Conrad Udo
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/s00425-004-1206-9
Published
2004-05-00
Epub
2004-00-07
Pages
158-66
Language
English
Region
Germany
NLM ID
1250576
Subset
IM
External Links
PubMed source DOI: 10.1007/s00425-004-1206-9 View journal details
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