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PMID: 18096623 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role of poly (A) tail as an identity element for mRNA nuclear export.

Nucleic acids research ·Vol. 36 ·No. 3 ·2008-02-00 ·Pages 1037-49

Fuke H, Ohno M

Abstract

Different RNA species are rigorously discriminated and exported by distinct export factors, but this discrimination mechanism remains largely unknown. We previously showed, by RNA microinjection experiments, that intronless mRNAs are discriminated from U snRNAs based on their difference in RNA length. However, it was unclear how they are discriminated in the natural situation in which their nascent transcripts emerge progressively during transcription. We hypothesized that transcription from the corresponding promoters is important for this discrimination. Here we show that contrary to our hypothesis, the discrimination process was not significantly influenced by whether transcription occurred from an mRNA- versus a U snRNA-type promoter. Rather, the features of transcribed RNAs determined the RNA identity, consistent with our previous results of RNA microinjection. Moreover, we found that the poly (A) tail can function as an identity element for mRNA export. The presence of a poly (A) tail of an appropriate length committed otherwise short Pol II transcripts to the mRNA export pathway in a dominant manner, indicating that the poly (A) tail either contributes to increasing the RNA length or functions as a platform to recruit mRNA export factors. Our results reveal a novel function of the poly (A) tail in mRNA export.

MeSH Terms
3' Untranslated Regions/chemistry Active Transport, Cell Nucleus Animals Cell Nucleus/metabolism Humans Poly A/chemistry,metabolism Polyadenylation Promoter Regions, Genetic RNA Transport RNA, Messenger/chemistry,metabolism RNA, Small Nuclear/chemistry,metabolism Regulatory Sequences, Ribonucleic Acid Transcription, Genetic Xenopus laevis
Chemicals
3' Untranslated Regions RNA, Messenger RNA, Small Nuclear Regulatory Sequences, Ribonucleic Acid Poly A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fuke Hiroyuki
Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.
Ohno Mutsuhito
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkm1120
Published
2008-02-00
Epub
2007-00-20
Pages
1037-49
Language
English
Region
England
NLM ID
0411011
PMCID
PMC2241894
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkm1120 PMC Full Text View journal details
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