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PMID: 15769879 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Yeast poly(A)-binding protein Pab1 shuttles between the nucleus and the cytoplasm and functions in mRNA export.

RNA (New York, N.Y.) ·Vol. 11 ·No. 4 ·2005-04-00 ·Pages 517-31

Brune C, Munchel SE, Fischer N, Podtelejnikov AV, Weis K

Abstract

Pab1 is the major poly(A)-binding protein in yeast. It is a multifunctional protein that mediates many cellular functions associated with the 3'-poly(A)-tail of messenger RNAs. Here, we characterize Pab1 as an export cargo of the protein export factor Xpo1/Crm1. Pab1 is a major Xpo1/Crm1-interacting protein in yeast extracts and binds directly to Xpo1/Crm1 in a RanGTP-dependent manner. Pab1 shuttles rapidly between the nucleus and the cytoplasm and partially accumulates in the nucleus when the function of Xpo1/Crm1 is inhibited. However, Pab1 can also be exported by an alternative pathway, which is dependent on the MEX67-mRNA export pathway. Import of Pab1 is mediated by the import receptor Kap108/Sxm1 through a nuclear localization signal in its fourth RNA-binding domain. Interestingly, inhibition of Pab1's nuclear import causes a kinetic delay in the export of mRNA. Furthermore, the inviability of a pab1 deletion strain is suppressed by a mutation in the 5'-3' exoribonuclease RRP6, a component of the nuclear exosome. Therefore, nuclear Pab1 may be required for efficient mRNA export and may function in the quality control of mRNA in the nucleus.

MeSH Terms
Active Transport, Cell Nucleus Cell Nucleus/metabolism Cytoplasm/metabolism Karyopherins/metabolism Nuclear Proteins/metabolism Nucleocytoplasmic Transport Proteins/metabolism Poly(A)-Binding Protein I/deficiency,genetics,metabolism RNA, Fungal/metabolism RNA, Messenger/metabolism RNA-Binding Proteins/metabolism Receptors, Cytoplasmic and Nuclear/metabolism Saccharomyces cerevisiae Proteins/metabolism Yeasts/genetics,metabolism
Chemicals
Karyopherins MEX67 protein, S cerevisiae Nuclear Proteins Nucleocytoplasmic Transport Proteins Poly(A)-Binding Protein I RNA, Fungal RNA, Messenger RNA-Binding Proteins Receptors, Cytoplasmic and Nuclear SXM1 protein, S cerevisiae Saccharomyces cerevisiae Proteins exportin 1 protein
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Brune Christiane
Department of Molecular and Cell Biology, Division of Cell and Developmental Biology, University of California, Berkeley, Berkeley, CA 94720-3200, USA.
Munchel Sarah E
Fischer Nicole
Podtelejnikov Alexandre V
Weis Karsten
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1261/rna.7291205
Published
2005-04-00
Pages
517-31
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1370741
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058065 · United States
NIGMS NIH HHS · R01 GM58065 · United States
External Links
PubMed source DOI: 10.1261/rna.7291205 PMC Full Text View journal details
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