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PMID: 10825193 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast Ran-binding protein 1 (Yrb1) shuttles between the nucleus and cytoplasm and is exported from the nucleus via a CRM1 (XPO1)-dependent pathway.

Molecular and cellular biology ·Vol. 20 ·No. 12 ·2000-06-00 ·Pages 4295-308

Künzler M, Gerstberger T, Stutz F, Bischoff FR, Hurt E

Abstract

The RanGTP-binding protein RanBP1, which is located in the cytoplasm, has been implicated in release of nuclear export complexes from the cytoplasmic side of the nuclear pore complex. Here we show that Yrb1 (the yeast homolog of RanBP1) shuttles between the nucleus and the cytoplasm. Nuclear import of Yrb1 is a facilitated process that requires a short basic sequence within the Ran-binding domain (RBD). By contrast, nuclear export of Yrb1 requires an intact RBD, which forms a ternary complex with the Xpo1 (Crm1) NES receptor in the presence of RanGTP. Nuclear export of Yrb1, however, is insensitive towards leptomycin B, suggesting a novel type of substrate recognition between Yrb1 and Xpo1. Taken together, these data suggest that ongoing nuclear import and export is an important feature of Yrb1 function in vivo.

MeSH Terms
Animals Biological Transport Carrier Proteins/metabolism Cell Nucleus/metabolism Cytoplasm/metabolism Fungal Proteins/metabolism Karyopherins Mice Nuclear Proteins/metabolism Receptors, Cytoplasmic and Nuclear Saccharomyces cerevisiae/metabolism
Chemicals
Carrier Proteins Fungal Proteins Karyopherins Nuclear Proteins Receptors, Cytoplasmic and Nuclear exportin 1 protein
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Künzler M
Ruprecht-Karls-Universität Heidelberg, Biochemie-Zentrum Heidelberg (BZH), Germany.
Gerstberger T
Stutz F
Bischoff F R
Hurt E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.20.12.4295-4308.2000
Published
2000-06-00
Pages
4295-308
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC85797
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.20.12.4295-4308.2000 PMC Full Text View journal details
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