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PMID: 11571268 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RanBP3 influences interactions between CRM1 and its nuclear protein export substrates.

EMBO reports ·Vol. 2 ·No. 10 ·2001-10-00 ·Pages 926-32

Englmeier L, Fornerod M, Bischoff FR, Petosa C, Mattaj IW, Kutay U

Abstract

We investigated the role of RanBP3, a nuclear member of the Ran-binding protein 1 family, in CRM1-mediated protein export in higher eukaryotes. RanBP3 interacts directly with CRM1 and also forms a trimeric complex with CRM1 and RanGTP. However, RanBP3 does not bind to CRM1 like an export substrate. Instead, it can stabilize CRM1-export substrate interaction. Nuclear RanBP3 stimulates CRM1-dependent protein export in permeabilized cells. These data indicate that RanBP3 functions by a novel mechanism as a cofactor in recognition and export of certain CRM1 substrates. In vitro, RanBP3 binding to CRM1 affects the relative affinity of CRM1 for different substrates.

MeSH Terms
Carrier Proteins/metabolism,physiology Cell Nucleus/metabolism Dose-Response Relationship, Drug GTP Phosphohydrolases/metabolism Glutathione Transferase/metabolism HeLa Cells Humans Karyopherins/chemistry,metabolism Kinetics Nuclear Proteins/metabolism,physiology Nucleocytoplasmic Transport Proteins Plasmids/metabolism Protein Binding Receptors, Cytoplasmic and Nuclear Substrate Specificity Time Factors ran GTP-Binding Protein/metabolism
Chemicals
Carrier Proteins Karyopherins Nuclear Proteins Nucleocytoplasmic Transport Proteins RANBP3 protein, human Receptors, Cytoplasmic and Nuclear exportin 1 protein Glutathione Transferase GTP Phosphohydrolases ran GTP-Binding Protein
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Englmeier L
European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Fornerod M
Bischoff F R
Petosa C
Mattaj I W
Kutay U
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Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-221X
DOI
10.1093/embo-reports/kve200
Published
2001-10-00
Epub
2001-00-24
Pages
926-32
Language
English
Region
England
NLM ID
100963049
PMCID
PMC1084078
Subset
IM
External Links
PubMed source DOI: 10.1093/embo-reports/kve200 PMC Full Text View journal details
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