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PMID: 12941705 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The 3' ends of human pre-snRNAs are produced by RNA polymerase II CTD-dependent RNA processing.

The EMBO journal ·Vol. 22 ·No. 17 ·2003-09-01 ·Pages 4544-54

Uguen P, Murphy S

Abstract

Proper 3' end formation of the human pre-snRNAs synthesized by pol II requires the cis-acting 3' box, although the precise function of this element has proved difficult to determine. In vivo, 3' end formation is tightly linked to transcription. However, we have now been able to obtain transcription-independent 3' box-dependent processing in vitro. This finally demonstrates that the 3' end of pre-snRNAs is produced by RNA processing rather than by termination of transcription. The phosphorylated form of the C-terminal domain (CTD) of pol II activates the processing event in vitro, consistent with our previous demonstration of the role of the CTD in pre-snRNA 3' end formation in vivo. In addition, we show that sequences upstream from the 3' box of the U2 snRNA gene influence 3' end formation both in vivo and in vitro.

MeSH Terms
Base Sequence Cations, Divalent/metabolism DNA/genetics HeLa Cells Humans In Vitro Techniques Models, Biological Nucleic Acid Conformation Protein Structure, Tertiary RNA Polymerase II/chemistry,genetics,metabolism RNA Precursors/chemistry,genetics,metabolism RNA Processing, Post-Transcriptional RNA, Small Nuclear/chemistry,genetics,metabolism Recombinant Fusion Proteins/chemistry,genetics,metabolism Transcription, Genetic
Chemicals
Cations, Divalent RNA Precursors RNA, Small Nuclear Recombinant Fusion Proteins U1 small nuclear RNA U2 small nuclear RNA DNA RNA Polymerase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Uguen Patricia
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
Murphy Shona
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg430
Published
2003-09-01
Pages
4544-54
Language
English
Region
England
NLM ID
8208664
PMCID
PMC202372
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdg430 PMC Full Text View journal details
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