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PMID: 6796940 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Primary and secondary structure of U2 snRNA.

Nucleic acids research ·Vol. 9 ·No. 21 ·1981-11-11 ·Pages 5645-58

Reddy R, Henning D, Epstein P, Busch H

Abstract

With the improved rapid sequencing techniques, the earlier sequence of U2 RNA of Novikoff hepatoma (Shibata et al, J. Biol. Chem. 250, 3909-3920, 1975) was reanalyzed and modified. The improved sequence of U2 RNA is 188 (or 189) nucleotides long and is in register with a characterized U2 RNA pseudogene (Denison et al, PNAS 78, 810-814, 1981) except for an 11 nucleotide sequence (nucleotides 147-157) which is absent from the pseudogene. From these results, a secondary structure of U2 RNA is proposed which is supported by the preferred cleavage sites with T1-RNase, RNase A and S1 nuclease. Isolated U2 RNA was cleaved by T1-RNase preferentially at positions 64 and 164, whereas U2 RNA in U2-snRNP was cleaved only at position 64, indicating that position 164 is protected in U2-snRNP. As with U1 RNA (Epstein et al, PNAS 78, 1562-1566, 1981) the 5'-end of isolated U2 RNA was not preferentially cleaved by T1-RNase.

MeSH Terms
Animals Base Sequence Liver Neoplasms, Experimental/analysis Nucleic Acid Conformation Oligoribonucleotides/analysis RNA, Neoplasm Rats Ribonuclease T1 Ribonucleases Ribonucleoproteins
Chemicals
Oligoribonucleotides RNA, Neoplasm Ribonucleoproteins Ribonucleases Ribonuclease T1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Reddy R
Henning D
Epstein P
Busch H
References (35)
35 references, click to expand
  1. Small molecular weight monodisperse nuclear RNA.
    J Mol Biol. 1968 Dec;38(3):289-304 PMID: 5718554
  2. The purine and pyrimidine composition of deoxypentose nucleic acids.
    Biochem J. 1951 May;48(5):584-90 PMID: 14838906
  3. Estimation of secondary structure in ribonucleic acids.
    Nature. 1971 Apr 9;230(5293):362-7 PMID: 4927725
  4. The sequence of 5 s ribosomal ribonucleic acid.
    J Mol Biol. 1968 Jun 28;34(3):379-412 PMID: 4938553
  5. MOdified nucleosides of nuclear and nucleolar low molecular weight ribonucleic acid.
    J Biol Chem. 1972 Nov 25;247(22):7245-50 PMID: 4344644
  6. Primary sequence of U-1 nuclear ribonucleic acid of Novikoff hepatoma ascites cells.
    J Biol Chem. 1974 Oct 25;249(20):6486-94 PMID: 4370679
  7. Low molecular weight nuclear RNA. The 3'-terminal sequence of the U2 RNA.
    Mol Cell Biochem. 1974 Aug 1;4(1):3-19 PMID: 4371257
  8. Evidence for the existence of a structural RNA component in the nuclear ribonucleoprotein particles containing heterogeneous RNA.
    Biochem Biophys Res Commun. 1975 Feb 3;62(3):642-50 PMID: 1120071
  9. The primary nucleotide sequence of nuclear U-2 ribonucleic acid. The 5'-terminal portion of the molecule.
    J Biol Chem. 1975 May 25;250(10):3909-20 PMID: 165188
  10. Sequence analysis of T1 ribonuclease fragments of 18S ribosomal RNA by 5'-terminal labeling, partial digestion, and homochromatography fingerprinting.
    Nucleic Acids Res. 1977 Feb;4(2):339-52 PMID: 190590
  11. Circular dichroism of ribonucleoprotein complexes from rat liver nuclei.
    Biochem Biophys Res Commun. 1977 Jun 20;76(4):1130-7 PMID: 901464
  12. Mapping adenines, guanines, and pyrimidines in RNA.
    Nucleic Acids Res. 1977 Aug;4(8):2527-38 PMID: 409999
  13. Computer analysis of nucleic acid regulatory sequences.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4401-5 PMID: 270683
  14. Occurrence of small molecular weight RNAs in Hela nuclear ribonucleoprotein particles containing HnRNA.
    Biochimie. 1977;59(8-9):755-8 PMID: 597505
  15. 3'-terminal labelling of RNA with T4 RNA ligase.
    Nature. 1978 Oct 12;275(5680):560-1 PMID: 692735
  16. Direct chemical method for sequencing RNA.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760-4 PMID: 377283
  17. An evaluation of small nuclear RNA in hnRNP.
    FEBS Lett. 1979 Aug 1;104(1):176-82 PMID: 477978
  18. Nucleotide sequence of nucleolar U3B RNA.
    J Biol Chem. 1979 Nov 10;254(21):11097-105 PMID: 500626
  19. Are snRNPs involved in splicing?
    Nature. 1980 Jan 10;283(5743):220-4 PMID: 7350545
  20. Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5495-9 PMID: 316537
  21. A mechanism for RNA splicing.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1877-9 PMID: 6246511
  22. Substitutions, insertions, and deletions in two highly conserved U3 RNA species.
    J Biol Chem. 1980 Jul 25;255(14):7029-33 PMID: 6771280
  23. The nucleotide sequence of nuclear U6 (4.7 S) RNA.
    J Biol Chem. 1980 Sep 25;255(18):8901-6 PMID: 6773955
  24. The nucleotide sequence of nuclear 4.8S RNA of mouse cells.
    Biochem Biophys Res Commun. 1980 Aug 14;95(3):1332-40 PMID: 6251836
  25. Nucleotide sequences of nuclear U1A RNAs from chicken, rat and man.
    Nucleic Acids Res. 1980 Sep 25;8(18):4143-54 PMID: 6159587
  26. The primary nucleotide sequence of U4 RNA.
    J Biol Chem. 1981 Apr 10;256(7):3532-8 PMID: 6162848
  27. Pseudouridine residues in the 5'-terminus of uridine-rich nuclear RNA I (U1 RNA).
    Biochem Biophys Res Commun. 1981 Feb 27;98(4):1076-83 PMID: 7225127
  28. The nuclear 5S RNAs from chicken, rat and man. U5 RNAs are encoded by multiple genes.
    Nucleic Acids Res. 1981 Feb 25;9(4):769-87 PMID: 6164980
  29. The conformation of chicken, rat and human U1A RNAs in solution.
    Nucleic Acids Res. 1981 Feb 25;9(4):841-58 PMID: 6164982
  30. Abundant pseudogenes for small nuclear RNAs are dispersed in the human genome.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):810-4 PMID: 6165010
  31. Site-specific cleavage by T1 RNase of U-1 RNA in u-1 ribonucleoprotein particles.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1562-6 PMID: 6165013
  32. Comparison of RNase T1 fingerprints of U1, U2, and U3 small nuclear RNA's of HeLa cells, human normal fibroblasts, and Novikoff hepatoma cells.
    Cancer Res. 1981 Jun;41(6):2215-20 PMID: 6165460
  33. Nucleotide sequence of nuclear 5S RNA of mouse cells.
    Biochem Biophys Res Commun. 1981 Apr 30;99(4):1468-76 PMID: 6167269
  34. Nucleotide sequence of nuclear 5.7S RNA of mouse cells.
    Biochem Biophys Res Commun. 1981 Apr 30;99(4):1477-85 PMID: 6167270
  35. Isolation and purification of a nuclear 4.4 S ribonucleic acid of the Novikoff hepatoma.
    J Biol Chem. 1970 Apr 25;245(8):1970-7 PMID: 4314936
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.21.5645
Published
1981-11-11
Pages
5645-58
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327549
Subset
IM
Grants
NCI NIH HHS · CA-10893 · United States
Databases
GENBANK
K00781
External Links
PubMed source DOI: 10.1093/nar/9.21.5645 PMC Full Text View journal details
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