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PMID: 18039830 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulatory Interactions among adhesin gene systems of uropathogenic Escherichia coli.

Infection and immunity ·Vol. 76 ·No. 2 ·2008-02-00 ·Pages 771-80

Lindberg S, Xia Y, Sondén B, Göransson M, Hacker J, Uhlin BE

Abstract

Uropathogenic Escherichia coli strain J96 carries multiple determinants for fimbrial adhesins. The regulatory protein PapB of P fimbriae has previously been implicated in potential coregulatory events. The focB gene of the F1C fimbria determinant is highly homologous to papB; the translated sequences share 81% identity. In this study we investigated the role of PapB and FocB in regulation of the F1C fimbriae. By using gel mobility shift assays, we showed that FocB binds to sequences in both the pap and foc operons in a somewhat different manner than PapB. The results of both in vitro cross-linking and in vivo oligomerization tests indicated that FocB could function in an oligomeric fashion. Furthermore, our results suggest that PapB and FocB can form heterodimers and that these complexes can repress expression of the foc operon. The effect of FocB on expression of type 1 fimbriae was also tested. Taken together, the results that we present expand our knowledge about a regulatory network for different adhesin gene systems in uropathogenic E. coli and suggest a hierarchy for expression of the fimbrial adhesins.

MeSH Terms
Adhesins, Bacterial/biosynthesis,genetics DNA, Bacterial/chemistry,metabolism DNA-Binding Proteins/genetics,metabolism Dimerization Electrophoretic Mobility Shift Assay Escherichia coli/genetics,metabolism Escherichia coli Proteins/metabolism Fimbriae Proteins/biosynthesis,genetics Gene Expression Regulation, Bacterial Membrane Proteins/metabolism Molecular Sequence Data Promoter Regions, Genetic Protein Binding Sequence Analysis, DNA Transcription Factors/metabolism
Chemicals
Adhesins, Bacterial DNA, Bacterial DNA-Binding Proteins Escherichia coli Proteins Membrane Proteins Transcription Factors atpD protein, E coli Fimbriae Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lindberg Stina
Department of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden, Umeå University, S-90187 Umeå, Sweden.
Xia Yan
Sondén Berit
Göransson Mikael
Hacker Jörg
Uhlin Bernt Eric
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
1098-5522
DOI
10.1128/IAI.01010-07
Published
2008-02-00
Epub
2007-00-26
Pages
771-80
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC2223471
Subset
IM
Databases
GENBANK
AM887937
External Links
PubMed source DOI: 10.1128/IAI.01010-07 PMC Full Text View journal details
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