Home LiteratureArticle Details
PMID: 10747013 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulatory cross-talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein.

The EMBO journal ·Vol. 19 ·No. 7 ·2000-04-03 ·Pages 1450-7

Xia Y, Gally D, Forsman-Semb K, Uhlin BE

Abstract

Pathogenic Escherichia coli often carry determinants for several different adhesins. We show a direct communication between two adhesin gene clusters in uropathogenic E.coli: type 1 fimbriae (fim) and pyelonephritis-associated pili (pap). A regulator of pap, PapB, is a key factor in this cross-talk. FimB recombinase turns on type 1 fimbrial expression, and PapB inhibited phase transition by FimB in both off-to-on and on-to-off directions. On-to-off switching requiring FimE was increased by PapB. By analysis of FimB- and FimE-LacZ translational fusions it was concluded that the increase in on-to-off transition rates was via an increase in FimE expression. Inhibition of FimB-promoted switching was via a different mechanism: PapB inhibited FimB-promoted in vitro recombination, indicating that FimB activity was blocked at the fim switch. In vitro analyses showed that PapB bound to several DNA regions of the type 1 fimbrial operon, including the fim switch region. These data show that Pap expression turns off type 1 fimbriae expression in the same cell. Such cross-talk between adhesin gene clusters may bring about appropriate expression at the single cell level.

MeSH Terms
Adhesins, Escherichia coli/genetics Bacterial Proteins/genetics Base Sequence DNA Primers/genetics DNA, Bacterial/genetics,metabolism DNA-Binding Proteins/genetics Escherichia coli/genetics,pathogenicity Escherichia coli Proteins Gene Expression Regulation, Bacterial Genes, Bacterial Genes, Switch Humans Integrases Membrane Proteins Multigene Family Operon Pili, Sex/genetics Promoter Regions, Genetic Recombination, Genetic Transcription Factors/genetics
Chemicals
Adhesins, Escherichia coli Bacterial Proteins DNA Primers DNA, Bacterial DNA-Binding Proteins Escherichia coli Proteins Membrane Proteins Transcription Factors atpD protein, E coli fimB protein, E coli fimE protein, E coli Integrases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Xia Y
Department of Microbiology, Umeå University, S-90187 Umeå, Sweden.
Gally D
Forsman-Semb K
Uhlin B E
References (37)
37 references, click to expand
  1. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  2. The leucine-responsive regulatory protein binds to the fim switch to control phase variation of type 1 fimbrial expression in Escherichia coli K-12.
    J Bacteriol. 1994 Sep;176(18):5665-72 PMID: 7916011
  3. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
    Gene. 1978 Oct;4(2):121-36 PMID: 363519
  4. Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate.
    Infect Immun. 1981 Sep;33(3):933-8 PMID: 6116675
  5. Contribution of adhesion to bacterial persistence in the mouse urinary tract.
    Infect Immun. 1983 Apr;40(1):265-72 PMID: 6131870
  6. Mutations in E coli cistrons affecting adhesion to human cells do not abolish Pap pili fiber formation.
    EMBO J. 1984 May;3(5):1159-65 PMID: 6145589
  7. Organization and expression of genes responsible for type 1 piliation in Escherichia coli.
    J Bacteriol. 1984 Aug;159(2):736-44 PMID: 6146599
  8. Immunofluorescence study of fimbrial phase variation in Escherichia coli KS71.
    J Bacteriol. 1984 Nov;160(2):691-5 PMID: 6150023
  9. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  10. An invertible element of DNA controls phase variation of type 1 fimbriae of Escherichia coli.
    Proc Natl Acad Sci U S A. 1985 Sep;82(17):5724-7 PMID: 2863818
  11. Two regulatory fim genes, fimB and fimE, control the phase variation of type 1 fimbriae in Escherichia coli.
    EMBO J. 1986 Jun;5(6):1389-93 PMID: 2874022
  12. Relationship of type 1 pilus expression in Escherichia coli to ascending urinary tract infections in mice.
    Infect Immun. 1987 Feb;55(2):373-80 PMID: 2879794
  13. Fimbrial phase variation in Escherichia coli: dependence on integration host factor and homologies with other site-specific recombinases.
    J Bacteriol. 1987 Aug;169(8):3840-3 PMID: 2886490
  14. Integration host factor is required for the DNA inversion that controls phase variation in Escherichia coli.
    Proc Natl Acad Sci U S A. 1987 Sep;84(18):6506-10 PMID: 2888114
  15. The Gal(alpha 1-4)Gal-specific tip adhesin of Escherichia coli P-fimbriae is needed for pyelonephritis to occur in the normal urinary tract.
    Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):11889-93 PMID: 7991552
  16. Type 1 fimbrial expression enhances Escherichia coli virulence for the urinary tract.
    Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9827-32 PMID: 8790416
  17. Interaction of FimB and FimE with the fim switch that controls the phase variation of type 1 fimbriae in Escherichia coli K-12.
    Mol Microbiol. 1996 Aug;21(4):725-38 PMID: 8878036
  18. Integration host factor stimulates both FimB- and FimE-mediated site-specific DNA inversion that controls phase variation of type 1 fimbriae expression in Escherichia coli.
    Mol Microbiol. 1997 Feb;23(4):705-17 PMID: 9157242
  19. Prevention of mucosal Escherichia coli infection by FimH-adhesin-based systemic vaccination.
    Science. 1997 Apr 25;276(5312):607-11 PMID: 9110982
  20. Promoter-specific repression of fimB expression by the Escherichia coli nucleoid-associated protein H-NS.
    J Bacteriol. 1997 Nov;179(21):6618-25 PMID: 9352908
  21. Leucine alters the interaction of the leucine-responsive regulatory protein (Lrp) with the fim switch to stimulate site-specific recombination in Escherichia coli.
    Mol Microbiol. 1998 Feb;27(4):751-61 PMID: 9515701
  22. Differential temperature modulation by H-NS of the fimB and fimE recombinase genes which control the orientation of the type 1 fimbrial phase switch.
    FEMS Microbiol Lett. 1998 May 1;162(1):17-23 PMID: 9595659
  23. Autoregulation and multiple DNA interactions by a transcriptional regulatory protein in E. coli pili biogenesis.
    EMBO J. 1989 Apr;8(4):1271-7 PMID: 2568258
  24. Improved vector system for constructing transcriptional fusions that ensures independent translation of lacZ.
    J Bacteriol. 1990 Feb;172(2):1077-84 PMID: 2137119
  25. Regulation of type 1 fimbrial expression in uropathogenic Escherichia coli: heterogeneity of expression through sequence changes in the fim switch region.
    Mol Microbiol. 1998 Apr;28(2):371-81 PMID: 9622361
  26. Induction and evasion of host defenses by type 1-piliated uropathogenic Escherichia coli.
    Science. 1998 Nov 20;282(5393):1494-7 PMID: 9822381
  27. Oligomeric interaction of the PapB transcriptional regulator with the upstream activating region of pili adhesin gene promoters in Escherichia coli.
    Mol Microbiol. 1998 Nov;30(3):513-23 PMID: 9822817
  28. Mutational analysis of the PapB transcriptional regulator in Escherichia coli. Regions important for DNA binding and oligomerization.
    J Biol Chem. 1999 Jul 9;274(28):19723-30 PMID: 10391913
  29. Upstream activating sequences that are shared by two divergently transcribed operons mediate cAMP-CRP regulation of pilus-adhesin in Escherichia coli.
    Mol Microbiol. 1989 Nov;3(11):1557-65 PMID: 2575704
  30. Rapid site-specific DNA inversion in Escherichia coli mutants lacking the histonelike protein H-NS.
    J Bacteriol. 1991 Jul;173(13):4116-23 PMID: 1648076
  31. Type 1 fimbriation and fimE mutants of Escherichia coli K-12.
    J Bacteriol. 1991 Sep;173(17):5298-307 PMID: 1679429
  32. Roles of fimB and fimE in site-specific DNA inversion associated with phase variation of type 1 fimbriae in Escherichia coli.
    J Bacteriol. 1991 Sep;173(17):5308-14 PMID: 1679430
  33. Lrp stimulates phase variation of type 1 fimbriation in Escherichia coli K-12.
    J Bacteriol. 1993 Jan;175(1):27-36 PMID: 8093239
  34. Inversion-independent phase variation of type 1 fimbriae in Escherichia coli.
    J Bacteriol. 1993 Jul;175(14):4335-44 PMID: 8101185
  35. Environmental regulation of the fim switch controlling type 1 fimbrial phase variation in Escherichia coli K-12: effects of temperature and media.
    J Bacteriol. 1993 Oct;175(19):6186-93 PMID: 8104927
  36. Localization of promoters in the fim gene cluster and the effect of H-NS on the transcription of fimB and fimE.
    FEMS Microbiol Lett. 1994 Feb 1;116(1):95-100 PMID: 7510658
  37. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.7.1450
Published
2000-04-03
Pages
1450-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC310214
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.7.1450 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2