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PMID: 8101185 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Inversion-independent phase variation of type 1 fimbriae in Escherichia coli.

Journal of bacteriology ·Vol. 175 ·No. 14 ·1993-07-00 ·Pages 4335-44

McClain MS, Blomfield IC, Eberhardt KJ, Eisenstein BI

Abstract

The roles of fimB and fimE in the phase-variable expression of type 1 fimbriae in Escherichia coli were examined. A method was developed to study the effects of fimB and fimE on both recombination of the fim invertible element and fimbrial expression. The method used an allelic exchange procedure consisting of two steps. The first step, construction of intermediate strains, deleted fimB and fimE. This step locked the invertible element in either the on or the off orientation. The second step of the exchange procedure introduced either wild-type or mutant alleles of fimB and/or fimE into the chromosome of the intermediate strains. Analysis of the resulting strains supported the current, plasmid-based model of recombination. Unexpectedly, strains in which the invertible element was locked in the on orientation (either by mutation of both fimB and fimE or, in a control strain, by mutation of the left inverted repeat sequence of the invertible element) continued to exhibit phase-variable expression of type 1 fimbriae. A strain in which fimA was transcribed from the tac promoter continued to exhibit phase-variable fimbrial expression, suggesting that inversion-independent phase variation cannot be explained by variable transcription initiation of fimA.

Related Genes
fimA fimB fimE
MeSH Terms
Alleles Bacterial Proteins/biosynthesis,genetics Base Sequence Chromosome Inversion Cloning, Molecular DNA, Bacterial/genetics,metabolism DNA, Recombinant/metabolism DNA-Binding Proteins/biosynthesis,genetics Escherichia coli/genetics,growth & development,physiology,ultrastructure Escherichia coli Proteins Fimbriae Proteins Fimbriae, Bacterial/physiology,ultrastructure Gene Expression Genes, Bacterial Integrases Microscopy, Electron Molecular Sequence Data Mutagenesis, Site-Directed Transcription, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Recombinant DNA-Binding Proteins Escherichia coli Proteins fimB protein, E coli fimE protein, E coli fimbrillin Fimbriae Proteins Integrases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
McClain M S
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620.
Blomfield I C
Eberhardt K J
Eisenstein B I
References (39)
39 references, click to expand
  1. Two modes of control of pilA, the gene encoding type 1 pilin in Escherichia coli.
    J Bacteriol. 1985 Oct;164(1):321-30 PMID: 3930469
  2. Characterization of repetitive sequences controlling phase variation of Haemophilus influenzae lipopolysaccharide.
    J Bacteriol. 1990 Jun;172(6):3304-9 PMID: 1693145
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. Role of chromosomal rearrangement in N. gonorrhoeae pilus phase variation.
    Cell. 1985 Feb;40(2):293-300 PMID: 2857113
  5. Phase variation of gonococcal pili by frameshift mutation in pilC, a novel gene for pilus assembly.
    EMBO J. 1991 Feb;10(2):477-88 PMID: 1671354
  6. Phase variation of type 1 fimbriae in Escherichia coli is under transcriptional control.
    Science. 1981 Oct 16;214(4518):337-9 PMID: 6116279
  7. Selective outgrowth of fimbriate bacteria in static liquid medium.
    J Bacteriol. 1970 Aug;103(2):447-56 PMID: 4914569
  8. Identification and characterization of a gene product that regulates type 1 piliation in Escherichia coli.
    J Bacteriol. 1984 Oct;160(1):61-6 PMID: 6148338
  9. Lectinophagocytosis: a molecular mechanism of recognition between cell surface sugars and lectins in the phagocytosis of bacteria.
    Infect Immun. 1988 Mar;56(3):539-47 PMID: 2893771
  10. Use of chromosomal gene fusions to investigate the role of repetitive DNA in regulation of genes involved in lipopolysaccharide biosynthesis in Haemophilus influenzae.
    J Bacteriol. 1992 Nov;174(22):7245-52 PMID: 1429450
  11. Nucleotide sequences of the genes encoding type 1 fimbrial subunits of Klebsiella pneumoniae and Salmonella typhimurium.
    J Bacteriol. 1987 Dec;169(12):5831-4 PMID: 2890624
  12. Genetic analysis of the phase variation control of expression of type 1 fimbriae in Escherichia coli.
    J Bacteriol. 1985 May;162(2):668-75 PMID: 2859269
  13. Analysis of recombinant DNA using Escherichia coli minicells.
    Methods Enzymol. 1983;101:347-62 PMID: 6350817
  14. Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.
    Gene. 1989 Apr 15;77(1):61-8 PMID: 2744488
  15. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 PMID: 3881765
  16. Specific insertion and deletion of insertion sequence 1-like DNA element causes the reversible expression of the virulence capsular antigen Vi of Citrobacter freundii in Escherichia coli.
    Proc Natl Acad Sci U S A. 1988 Jun;85(12):4402-5 PMID: 2837765
  17. MINIATURE escherichia coli CELLS DEFICIENT IN DNA.
    Proc Natl Acad Sci U S A. 1967 Feb;57(2):321-6 PMID: 16591472
  18. Type 1 fimbriae mutants of Escherichia coli K12: characterization of recognized afimbriate strains and construction of new fim deletion mutants.
    Mol Microbiol. 1991 Jun;5(6):1439-45 PMID: 1686292
  19. Identification of a sex-factor-affinity site in E. coli as gamma delta.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:135-40 PMID: 6271456
  20. Pseudocatabolite repression of type 1 fimbriae of Escherichia coli.
    J Bacteriol. 1982 Sep;151(3):1560-7 PMID: 6125501
  21. Isolation and characterization of mutants with lesions affecting pellicle formation and erythrocyte agglutination by type 1 piliated Escherichia coli.
    J Bacteriol. 1990 Nov;172(11):6411-8 PMID: 1977736
  22. Phase variation of gonococcal protein II: regulation of gene expression by slipped-strand mispairing of a repetitive DNA sequence.
    Cell. 1989 Feb 24;56(4):539-47 PMID: 2492905
  23. Two regulatory fim genes, fimB and fimE, control the phase variation of type 1 fimbriae in Escherichia coli.
    EMBO J. 1986 Jun;5(6):1389-93 PMID: 2874022
  24. Regulation of pap pilin phase variation by a mechanism involving differential dam methylation states.
    EMBO J. 1990 Dec;9(12):4045-54 PMID: 2147413
  25. Synthetic sites for transcription termination and a functional comparison with tryptophan operon termination sites in vitro.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4180-4 PMID: 7027254
  26. An invertible element of DNA controls phase variation of type 1 fimbriae of Escherichia coli.
    Proc Natl Acad Sci U S A. 1985 Sep;82(17):5724-7 PMID: 2863818
  27. Roles of fimB and fimE in site-specific DNA inversion associated with phase variation of type 1 fimbriae in Escherichia coli.
    J Bacteriol. 1991 Sep;173(17):5308-14 PMID: 1679430
  28. Purification of the bacteriophage lambda xis gene product required for lambda excisive recombination.
    J Biol Chem. 1982 Aug 25;257(16):9658-62 PMID: 6213611
  29. Isolation and characterization of a monoclonal antibody directed against type 1 fimbriae organelles from Escherichia coli.
    Infect Immun. 1983 Oct;42(1):333-40 PMID: 6194117
  30. Inability of toxin inhibitors to neutralize enhanced toxicity caused by bacteria adherent to tissue culture cells.
    Infect Immun. 1990 Nov;58(11):3737-42 PMID: 1699898
  31. Effect of Tamm-Horsfall urinary glycoprotein on phagocytosis and killing of type I-fimbriated Escherichia coli.
    Infect Immun. 1986 Jan;51(1):193-8 PMID: 2867043
  32. The tac promoter: a functional hybrid derived from the trp and lac promoters.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):21-5 PMID: 6337371
  33. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon.
    Mol Microbiol. 1991 Jun;5(6):1447-57 PMID: 1686293
  34. Regulation of the phase switch controlling expression of type 1 fimbriae in Escherichia coli.
    Mol Microbiol. 1989 Jul;3(7):925-31 PMID: 2571915
  35. Culture medium for enterobacteria.
    J Bacteriol. 1974 Sep;119(3):736-47 PMID: 4604283
  36. Growth advantage and enhanced toxicity of Escherichia coli adherent to tissue culture cells due to restricted diffusion of products secreted by the cells.
    J Clin Invest. 1987 Apr;79(4):1210-6 PMID: 3031133
  37. Type 1 fimbriation and fimE mutants of Escherichia coli K-12.
    J Bacteriol. 1991 Sep;173(17):5298-307 PMID: 1679429
  38. Characterization of two genes encoding antigenically distinct type-1 fimbriae of Klebsiella pneumoniae.
    Gene. 1988 Apr 29;64(2):231-40 PMID: 2456966
  39. Lrp stimulates phase variation of type 1 fimbriation in Escherichia coli K-12.
    J Bacteriol. 1993 Jan;175(1):27-36 PMID: 8093239
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.14.4335-4344.1993
Published
1993-07-00
Pages
4335-44
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204873
Subset
IM
Grants
NIAID NIH HHS · 5T32 AI07360 · United States
NIAID NIH HHS · AI24734 · United States
External Links
PubMed source DOI: 10.1128/jb.175.14.4335-4344.1993 PMC Full Text View journal details
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