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PMID: 2890081 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Three fim genes required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae.

Molecular & general genetics : MGG ·Vol. 208 ·No. 3 ·1987-07-00 ·Pages 439-45

Klemm P, Christiansen G

Abstract

Three novel fim genes of Escherichia coli, fimF, fimG and fimH, were characterized. These genes were not necessary for the production of fimbriae but were shown to be involved in the adhesive property and longitudinal regulation of these structures. Complementation experiments indicated that both the major fimbrial subunit gene, fimA, and the fimH gene in combination with either the fimF or the fimG gene were required for mannose-specific adhesion. The fimF, fimG and fimH gene products were likewise shown to play a major role in the fimbrial morphology as longitudinal modulators. The amount of FimF, FimG and FimH proteins appeared to control the length and number of the fimbriae. The DNA sequence of a 2050 bp region containing the three genes was determined. The corresponding protein sequences all exhibited homology with the fimbrial subunit protein, FimA.

MeSH Terms
Amino Acid Sequence Bacterial Adhesion Bacterial Proteins/genetics Base Sequence DNA Restriction Enzymes Escherichia coli/genetics,physiology,ultrastructure Escherichia coli Proteins/genetics Fimbriae Proteins/genetics Fimbriae, Bacterial/physiology,ultrastructure Genes Genes, Bacterial Genetic Complementation Test Molecular Sequence Data Plasmids
Chemicals
Bacterial Proteins Escherichia coli Proteins FimG protein, E coli Fimbriae Proteins DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Klemm P
Department of Microbiology, Technical University of Denmark, Lyngby.
Christiansen G
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00328136
Published
1987-07-00
Pages
439-45
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X05672
External Links
PubMed source DOI: 10.1007/BF00328136
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