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PMID: 17878950 Published · epublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Connector inversion probe technology: a powerful one-primer multiplex DNA amplification system for numerous scientific applications.

PloS one ·Vol. 2 ·No. 9 ·2007-09-19 ·Pages e915

Akhras MS, Unemo M, Thiyagarajan S, Nyrén P, Davis RW, Fire AZ, Pourmand N

Abstract

We combined components of a previous assay referred to as Molecular Inversion Probe (MIP) with a complete gap filling strategy, creating a versatile powerful one-primer multiplex amplification system. As a proof-of-concept, this novel method, which employs a Connector Inversion Probe (CIPer), was tested as a genetic tool for pathogen diagnosis, typing, and antibiotic resistance screening with two distinct systems: i) a conserved sequence primer system for genotyping Human Papillomavirus (HPV), a cancer-associated viral agent and ii) screening for antibiotic resistance mutations in the bacterial pathogen Neisseria gonorrhoeae. We also discuss future applications and advances of the CIPer technology such as integration with digital amplification and next-generation sequencing methods. Furthermore, we introduce the concept of two-dimension informational barcodes, i.e. "multiplex multiplexing padlocks" (MMPs). For the readers' convenience, we also provide an on-line tutorial with user-interface software application CIP creator 1.0.1, for custom probe generation from virtually any new or established primer-pairs.

MeSH Terms
DNA/genetics DNA Probes Drug Resistance, Microbial/genetics Mutation
Chemicals
DNA Probes DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Akhras Michael S
Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America.
Unemo Magnus
Thiyagarajan Sreedevi
Nyrén Pål
Davis Ronald W
Fire Andrew Z
Pourmand Nader
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0000915
Published
2007-09-19
Epub
2007-00-19
Pages
e915
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC1976392
Subset
IM
Grants
NHGRI NIH HHS · P01 HG000205 · United States
NHGRI NIH HHS · P01-HG000205 · United States
External Links
PubMed source DOI: 10.1371/journal.pone.0000915 PMC Full Text View journal details
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