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PMID: 17367469 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular characterization of Neisseria gonorrhoeae identifies transmission and resistance of one ciprofloxacin-resistant strain.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica ·Vol. 115 ·No. 3 ·2007-03-00 ·Pages 231-41

Unemo M, Sjöstrand A, Akhras M, Gharizadeh B, Lindbäck E, Pourmand N, Wretlind B, Fredlund H

Abstract

A highly discriminative and objective genetic characterization of N. gonorrhoeae, which increases our knowledge of strain populations in different geographic areas, is crucial for the development of improved control measures. In the present study, conventional phenotypic characterization and genetic characterization by means of pulsed-field gel electrophoresis (PFGE), sequencing of the entire porB gene, N. gonorrhoeae multiantigen sequence typing (NG-MAST), and pyrosequencing of a quinolone resistance determining region (QRDR) of the gyrA gene of Swedish ciprofloxacin-resistant N. gonorrhoeae serovar IB-10 isolates (n=45) were performed. The genetic characterization identified one widely spread ciprofloxacin-resistant N. gonorrhoeae ST147 strain. In addition, isolates with slightly different genetic characteristics, which presumably reflect the ongoing evolution only, were also identified. All the isolates contained single nucleotide polymorphisms in QRDR of the gyrA gene that are highly correlated with ciprofloxacin resistance. Consequently, comprehensive characterization identified the first confirmed large domestic transmission, mainly among young heterosexuals, of one ciprofloxacin-resistant N. gonorrhoeae strain in Swedish society during 2002-2003. In conclusion, a precise, i.e. genetic, characterization for identification of individual strains is a very valuable support to the crucial active surveillance of the epidemiological characteristics and the antibiotic susceptibility of N. gonorrhoeae in the effective treatment of gonorrhoea.

MeSH Terms
Ciprofloxacin/pharmacology,therapeutic use Drug Resistance, Bacterial Gonorrhea/drug therapy,epidemiology,transmission Humans Mandatory Reporting Microbial Sensitivity Tests Neisseria gonorrhoeae/classification,drug effects,genetics Phylogeny Sensitivity and Specificity Sweden/epidemiology
Chemicals
Ciprofloxacin
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Unemo Magnus
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden. magnus.unemo@orebroll.se
Sjöstrand Anneli
Akhras Michael
Gharizadeh Baback
Lindbäck Emma
Pourmand Nader
Wretlind Bengt
Fredlund Hans
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Article Info
Journal
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
Abbr.
APMIS
ISSN
0903-4641
DOI
10.1111/j.1600-0463.2007.apm_487.x
Email
magnus.unemo@orebroll.se
Published
2007-03-00
Pages
231-41
Language
English
Region
Denmark
NLM ID
8803400
PMCID
PMC2769514
Subset
IM
Grants
NHGRI NIH HHS · P01 HG000205 · United States
NHGRI NIH HHS · P01 HG000205-190004 · United States
External Links
PubMed source DOI: 10.1111/j.1600-0463.2007.apm_487.x PMC Full Text View journal details
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