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PMID: 17646381 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Assembly of regulatory factors on rRNA and ribosomal protein genes in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 27 ·No. 19 ·2007-10-00 ·Pages 6686-705

Kasahara K, Ohtsuki K, Ki S, Aoyama K, Takahashi H, Kobayashi T, Shirahige K, Kokubo T

Abstract

HMO1 is a high-mobility group B protein that plays a role in transcription of genes encoding rRNA and ribosomal proteins (RPGs) in Saccharomyces cerevisiae. This study uses genome-wide chromatin immunoprecipitation to study the roles of HMO1, FHL1, and RAP1 in transcription of these genes as well as other RNA polymerase II-transcribed genes in yeast. The results show that HMO1 associates with the 35S rRNA gene in an RNA polymerase I-dependent manner and that RPG promoters (138 in total) can be classified into several distinct groups based on HMO1 abundance at the promoter and the HMO1 dependence of FHL1 and/or RAP1 binding to the promoter. FHL1, a key regulator of RPGs, binds to most of the HMO1-enriched and transcriptionally HMO1-dependent RPG promoters in an HMO1-dependent manner, whereas it binds to HMO1-limited RPG promoters in an HMO1-independent manner, irrespective of whether they are transcribed in an HMO1-dependent manner. Reporter gene assays indicate that these functional properties are determined by the promoter sequence.

MeSH Terms
DNA-Binding Proteins/genetics,metabolism Forkhead Transcription Factors Gene Expression Regulation, Fungal Genome, Fungal High Mobility Group Proteins/genetics,metabolism Promoter Regions, Genetic RNA Polymerase I/metabolism RNA, Ribosomal/genetics,metabolism Ribosomal Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Shelterin Complex Telomere-Binding Proteins/genetics,metabolism Transcription Factors/genetics,metabolism
Chemicals
DNA-Binding Proteins FHL1 protein, S cerevisiae Forkhead Transcription Factors HMO1 protein, S cerevisiae High Mobility Group Proteins RAP1 protein, S cerevisiae RNA, Ribosomal Ribosomal Proteins Saccharomyces cerevisiae Proteins Shelterin Complex Telomere-Binding Proteins Transcription Factors RNA Polymerase I
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Kasahara Koji
Division of Molecular and Cellular Biology, Science of Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University, Yokohama, Kanagawa, Japan.
Ohtsuki Kazushige
Ki Sewon
Aoyama Kayo
Takahashi Hiroyuki
Kobayashi Takehiko
Shirahige Katsuhiko
Kokubo Tetsuro
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.00876-07
Published
2007-10-00
Epub
2007-00-23
Pages
6686-705
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC2099245
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.00876-07 PMC Full Text View journal details
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