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PMID: 1756727 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcriptional termination between the closely linked human complement genes C2 and factor B: common termination factor for C2 and c-myc?

The EMBO journal ·Vol. 10 ·No. 13 ·1991-12-00 ·Pages 4197-207

Ashfield R, Enriquez-Harris P, Proudfoot NJ

Abstract

We have demonstrated, using a combination of nuclear run-off and poly(A) site competition assays, that transcriptional termination occurs between the closely spaced human complement genes, C2 and Factor B, soon after the C2 poly(A) site. A comparison of the C2 termination signal with a functionally similar sequence downstream of the human alpha 2 globin gene reveals that both signals function in an orientation dependent manner, with subfragments of the whole signal displaying partial effects. In the case of the C2 termination sequence a protein binds within it, and is partially responsible for the termination effect. We further demonstrate that the same (or closely related) protein binds to the ME1a1 site in the murine c-myc promoter, which has been implicated in c-myc attenuation. We suggest that the termination/pause sequences positioned downstream of a gene's poly(A) site may constitute the general signals that elicit transcriptional termination in genes transcribed by RNA polymerase II.

MeSH Terms
Base Sequence Chloramphenicol O-Acetyltransferase/metabolism Complement C2/genetics Complement Factor B/genetics DNA Fingerprinting Globins/genetics,metabolism Humans Liver/enzymology,metabolism Molecular Sequence Data Plasmids Promoter Regions, Genetic Proto-Oncogene Proteins c-myc/genetics Restriction Mapping Sequence Homology, Nucleic Acid Terminator Regions, Genetic Transfection beta-Galactosidase/metabolism
Chemicals
Complement C2 Proto-Oncogene Proteins c-myc Globins Chloramphenicol O-Acetyltransferase beta-Galactosidase Complement Factor B
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ashfield R
Sir William Dunn School of Pathology, University of Oxford, UK.
Enriquez-Harris P
Proudfoot N J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-12-00
Pages
4197-207
Language
English
Region
England
NLM ID
8208664
PMCID
PMC453172
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source PMC Full Text View journal details
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