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PMID: 175234 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cholesteryl ester metabolism in tissue culture cells: II. Source of accumulated esterified cholesterol in Fu5AH rat hepatoma cells.

Lipids ·Vol. 11 ·No. 2 ·1976-02-00 ·Pages 97-108

Rothblat GH, Arbogast L, Kritchevsky D, Naftulin M

Abstract

Previous investigations had demonstrated that Fu5AH rat hepatoma cells accumulated large quantities of esterified cholesterol when grown in hyperlipemic rabbit serum. The present investigation has determined the sources of the cellular esterified cholesterol when the cells were grown in hyperlipemic serum. Cellular esterification of endogenous and exogenous free cholesterol contributed 10% and 30%, respectively. The remaining 60% of the accumulated cellular esterfied cholesterol was derived from exogenous (serum) cholesteryl esters. Evidence for the hydrolysis of a portion of the incorporated esterified cholesterol is presented. A stimulation of free cholesterol incorporation and cellular esterification is elicited by hyperlipemic serum and serum lipoproteins when compared to normolipemic serum present at equivalent exogenous cholesterol concentrations. The effect of hyperlipemic serum is reduced by Tween-80 and Triton WR-1339. Comparative data on esterified cholesterol accumulation, free cholesterol incorporation, and cellular cholesterol esterification in Fu5-5 rat hepatoma cells, L-cells, and rabbit aortic medial cells are presented.

MeSH Terms
Animals Aorta/cytology,drug effects Blood Carcinoma, Hepatocellular/metabolism Cholesterol/analogs & derivatives,metabolism,pharmacology Cholesterol Esters/blood,metabolism,pharmacology Culture Media Culture Techniques Detergents/pharmacology Dietary Fats Hyperlipidemias/blood L Cells/drug effects Lipids/pharmacology Lipoproteins/metabolism Liver Neoplasms/metabolism Rabbits
Chemicals
Cholesterol Esters Culture Media Detergents Dietary Fats Lipids Lipoproteins Cholesterol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rothblat G H
Arbogast L
Kritchevsky D
Naftulin M
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48 references, click to expand
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Article Info
Journal
Lipids
Abbr.
Lipids
ISSN
0024-4201
DOI
10.1007/BF02532658
Published
1976-02-00
Pages
97-108
Language
English
Region
United States
NLM ID
0060450
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02532658 View journal details
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