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PMID: 17510367 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Conformational switching in the fungal light sensor Vivid.

Science (New York, N.Y.) ·Vol. 316 ·No. 5827 ·2007-05-18 ·Pages 1054-7

Zoltowski BD, Schwerdtfeger C, Widom J, Loros JJ, Bilwes AM, Dunlap JC, Crane BR

Abstract

The Neurospora crassa photoreceptor Vivid tunes blue-light responses and modulates gating of the circadian clock. Crystal structures of dark-state and light-state Vivid reveal a light, oxygen, or voltage Per-Arnt-Sim domain with an unusual N-terminal cap region and a loop insertion that accommodates the flavin cofactor. Photoinduced formation of a cystein-flavin adduct drives flavin protonation to induce an N-terminal conformational change. A cysteine-to-serine substitution remote from the flavin adenine dinucleotide binding site decouples conformational switching from the flavin photocycle and prevents Vivid from sending signals in Neurospora. Key elements of this activation mechanism are conserved by other photosensors such as White Collar-1, ZEITLUPE, ENVOY, and flavin-binding, kelch repeat, F-BOX 1 (FKF1).

MeSH Terms
Adaptation, Physiological Amino Acid Sequence Amino Acid Substitution Binding Sites Crystallography, X-Ray Darkness Dimerization Flavin-Adenine Dinucleotide/chemistry Fungal Proteins/chemistry,genetics,metabolism Light Molecular Sequence Data Mutagenesis Neurospora crassa/chemistry Protein Conformation Protein Structure, Tertiary
Chemicals
Fungal Proteins VVD protein, Neurospora crassa Flavin-Adenine Dinucleotide
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Zoltowski Brian D
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA.
Schwerdtfeger Carsten
Widom Joanne
Loros Jennifer J
Bilwes Alexandrine M
Dunlap Jay C
Crane Brian R
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1137128
Published
2007-05-18
Pages
1054-7
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC3682417
Subset
IM
Grants
NIGMS NIH HHS · R01 GM034985 · United States
NIGMS NIH HHS · R37GM34985 · United States
NIGMS NIH HHS · R37 GM034985 · United States
NIMH NIH HHS · R01 MH044651 · United States
NIGMS NIH HHS · P01 GM068087 · United States
NIGMS NIH HHS · F31 GM079879 · United States
NIGMS NIH HHS · GM079879-01 · United States
NIGMS NIH HHS · R01 GM034985-24 · United States
NIMH NIH HHS · MH44651 · United States
Databases
PDB
2PD7, 2PD8, 2PDR, 2PDT
External Links
PubMed source DOI: 10.1126/science.1137128 PMC Full Text View journal details
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