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PMID: 17504447 Published · ppublish English Journal Article

Reduced protein expression of metastasis-related genes (nm23, KISS1, KAI1 and p53) in lymph node and liver metastases of gastric cancer.

International journal of experimental pathology ·Vol. 88 ·No. 3 ·2007-06-00 ·Pages 175-83

Guan-Zhen Y, Ying C, Can-Rong N, Guo-Dong W, Jian-Xin Q, Jie-Jun W

Abstract

Metastasis remains an incurable common complication in patients with gastric cancer. A variety of theories have been proposed to explain the inefficiency of the metastatic process. To compare protein expression of metastasis-related genes (nm23, KISS1, KAI1 and p53) between primary tumours and metastatic tumours may be useful in illustrating these theories. Metastasis-related tissue microarrays (including normal tissues, primary tumours, nodal metastases and liver metastases) were constructed. The protein expression of nm23, KISS1, KAI1 and p53 in lymph node and liver metastases from advanced gastric cancer specimens was mainly examined by immunohistochemical staining in relation to primary tumours. Immunohistochemical staining showed reduced protein expression of nm23, KISS1 and KAI1 in lymph node and liver metastases compared with primary tumours. Results for p53 were to the contrary. Our investigations revealed a tendency of reduced protein expression of metastasis suppressor genes nm23, KISS1 and KAI1 in gastric cancer with the progress of metastasis. This means that the progression theory is an important determinant of metastatic efficiency.

MeSH Terms
Adult Aged Aged, 80 and over Biomarkers, Tumor/analysis Female Gene Expression Gene Expression Profiling Genetic Markers Humans Immunohistochemistry Kangai-1 Protein/analysis,genetics Kisspeptins Liver Neoplasms/metabolism,secondary Lymphatic Metastasis Male Middle Aged NM23 Nucleoside Diphosphate Kinases Nucleoside-Diphosphate Kinase/analysis,genetics Oligonucleotide Array Sequence Analysis Staining and Labeling Stomach Neoplasms/metabolism Tumor Suppressor Protein p53/analysis,genetics Tumor Suppressor Proteins/analysis,genetics
Chemicals
Biomarkers, Tumor Genetic Markers KISS1 protein, human Kangai-1 Protein Kisspeptins NM23 Nucleoside Diphosphate Kinases Tumor Suppressor Protein p53 Tumor Suppressor Proteins NME1 protein, human Nucleoside-Diphosphate Kinase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Guan-Zhen Yu
Department of Oncology, Changzheng Hospital, Shanghai, China.
Ying Chen
Can-Rong Ni
Guo-Dong Wang
Jian-Xin Qian
Jie-Jun Wang
References (40)
40 references, click to expand
  1. Reduced expression of the metastasis suppressor gene KAI1 in advanced colon cancer and its metastases.
    Surgery. 1999 Nov;126(5):869-80 PMID: 10568187
  2. Correlation of KAI1/CD82 gene expression with good prognosis in patients with non-small cell lung cancer.
    Cancer Res. 1996 Apr 15;56(8):1751-5 PMID: 8620488
  3. Aberration of p53 and DCC in gastric and colorectal cancer.
    Oncol Rep. 2000 Jan-Feb;7(1):99-103 PMID: 10601600
  4. KAI1 protein is down-regulated during the progression of human breast cancer.
    Clin Cancer Res. 2000 Sep;6(9):3424-9 PMID: 10999724
  5. Is the KAI1 metastasis suppressor gene a cellular target of p53? A review of current evidence.
    Biochem Biophys Res Commun. 2000 Nov 30;278(3):499-502 PMID: 11095940
  6. KiSS-1 represses 92-kDa type IV collagenase expression by down-regulating NF-kappa B binding to the promoter as a consequence of Ikappa Balpha -induced block of p65/p50 nuclear translocation.
    J Biol Chem. 2001 Jan 12;276(2):1164-72 PMID: 11060311
  7. nm23 protein expression in colorectal carcinoma metastasis in regional lymph nodes and the liver.
    Eur J Surg Oncol. 2001 Mar;27(2):170-4 PMID: 11289754
  8. Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor.
    Nature. 2001 May 31;411(6837):613-7 PMID: 11385580
  9. Overexpression of KAI1 suppresses in vitro invasiveness and in vivo metastasis in breast cancer cells.
    Cancer Res. 2001 Jul 1;61(13):5284-8 PMID: 11431371
  10. The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54.
    J Biol Chem. 2001 Sep 14;276(37):34631-6 PMID: 11457843
  11. Transduction of KAI1/CD82 cDNA promotes hematogenous spread of human lung-cancer cells in natural killer cell-depleted SCID mice.
    Int J Cancer. 2001 Oct 1;94(1):16-23 PMID: 11668473
  12. [Expression of nm23 in breast cancer: correlation with distant metastasis and prognosis].
    Zhonghua Zhong Liu Za Zhi. 2001 May;23(3):224-7 PMID: 11783092
  13. Genetic basis of human breast cancer metastasis.
    J Mammary Gland Biol Neoplasia. 2001 Oct;6(4):441-51 PMID: 12013533
  14. Potential role and chronology of abnormal expression of the Deleted in Colon Cancer (DCC) and the p53 proteins in the development of gastric cancer.
    BMC Cancer. 2001;1:9 PMID: 11518545
  15. Prognostic implication of nm23-H1 expression in colorectal carcinomas.
    Pathology. 2002 Oct;34(5):427-32 PMID: 12408341
  16. Tumor suppressor role of KiSS-1 in bladder cancer: loss of KiSS-1 expression is associated with bladder cancer progression and clinical outcome.
    Am J Pathol. 2003 Feb;162(2):609-17 PMID: 12547718
  17. KISS1 metastasis suppression and emergent pathways.
    Clin Exp Metastasis. 2003;20(1):11-8 PMID: 12650602
  18. Effects of KAI1/CD82 on biological behavior of human colorectal carcinoma cell line.
    World J Gastroenterol. 2003 Jun;9(6):1231-6 PMID: 12800230
  19. Knockout mice as model systems for studying nm23/NDP kinase gene functions. Application to the nm23-M1 gene.
    J Bioenerg Biomembr. 2003 Feb;35(1):19-30 PMID: 12848338
  20. Nm23-H1 expression does not predict clinical survival in colorectal cancer patients.
    Oncol Rep. 2003 Sep-Oct;10(5):1257-63 PMID: 12883690
  21. nm23-H1 protein immunoreactivity in laryngeal carcinoma.
    Cancer. 1996 Jun 1;77(11):2246-50 PMID: 8635091
  22. Expression of p53 and NDP-K/nm23 in gastric carcinomas--association with metastasis and clinicopathologic parameters.
    J Korean Med Sci. 1995 Dec;10(6):406-13 PMID: 8924224
  23. The prediction of lymph node metastases in colorectal cancer by expression of the nucleoside diphosphate kinase/nm23-H1 and histopathological variables.
    Am J Gastroenterol. 1997 Jul;92(7):1182-6 PMID: 9219795
  24. Nm23 gene expression in gastric carcinoma: an immunohistochemical study.
    Aust N Z J Surg. 1998 Mar;68(3):180-2 PMID: 9563444
  25. Distant metastasis of gastric cancer is associated with elevated expression of the antimetastatic nm23 gene.
    Cancer Lett. 1998 Jun 5;128(1):23-9 PMID: 9652789
  26. The expression of the KAI1 gene, a tumor metastasis suppressor, is directly activated by p53.
    Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11307-11 PMID: 9736732
  27. nm23 in the primary and metastatic sites of gastric carcinoma. Relation to AFP-producing carcinoma.
    Oncology. 1999;56(2):122-8 PMID: 9949298
  28. Protein markers in colorectal cancer: predictors of liver metastasis.
    Ann Surg. 1999 Aug;230(2):179-84 PMID: 10450731
  29. Reduced metastasis-suppressor gene mRNA-expression in breast cancer brain metastases.
    J Cancer Res Clin Oncol. 2005 Mar;131(3):191-8 PMID: 15592684
  30. Papillary microcarcinomas of the thyroid with preoperatively detectable lymph node metastasis show significantly higher aggressive characteristics on immunohistochemical examination.
    Oncology. 2005;68(2-3):87-96 PMID: 15886500
  31. Quantitative reverse transcriptase polymerase chain reaction analysis for KiSS-1 and orphan G-protein-coupled receptor (hOT7T175) gene expression in hepatocellular carcinoma.
    J Cancer Res Clin Oncol. 2003 Sep;129(9):531-5 PMID: 12898236
  32. Allelic diversity in the host genetic background may be an important determinant in tumor metastatic dissemination.
    Cancer Lett. 2003 Oct 28;200(2):97-105 PMID: 14568162
  33. nm23-H1 reduces in vitro cell migration and the liver metastatic potential of colon cancer cells by regulating myosin light chain phosphorylation.
    Int J Cancer. 2004 Jan 10;108(2):207-11 PMID: 14639604
  34. Clinical significance of the loss of KiSS-1 and orphan G-protein-coupled receptor (hOT7T175) gene expression in esophageal squamous cell carcinoma.
    Clin Cancer Res. 2004 Feb 15;10(4):1379-83 PMID: 14977840
  35. Expression of ATM protein and its relationship with p53 in pancreatic carcinoma with tissue array.
    Pancreas. 2004 May;28(4):421-6 PMID: 15097860
  36. Co-downregulation of PTEN, KAI-1, and nm23-H1 tumor/metastasis suppressor proteins in non-small cell lung cancer.
    Ann Diagn Pathol. 2004 Feb;8(1):6-16 PMID: 15129904
  37. Downregulation of KiSS-1 expression is responsible for tumor invasion and worse prognosis in gastric carcinoma.
    Int J Cancer. 2004 Oct 10;111(6):868-72 PMID: 15300798
  38. Gastric cancer with p53 overexpression has high potential for metastasising to lymph nodes.
    Br J Cancer. 1993 Mar;67(3):589-93 PMID: 8439509
  39. A novel function for the nm23-H1 gene: overexpression in human breast carcinoma cells leads to the formation of basement membrane and growth arrest.
    J Natl Cancer Inst. 1994 Dec 21;86(24):1838-44 PMID: 7990158
  40. Loss of KAI1 expression in the progression of colorectal cancer.
    Cancer Res. 1999 Nov 15;59(22):5724-31 PMID: 10582691
Article Info
Journal
International journal of experimental pathology
Abbr.
Int J Exp Pathol
ISSN
0959-9673
DOI
10.1111/j.1365-2613.2006.00510.x
Published
2007-06-00
Pages
175-83
Language
English
Region
England
NLM ID
9014042
PMCID
PMC2517304
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1365-2613.2006.00510.x PMC Full Text View journal details
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