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PMID: 15592684 Published · ppublish English Journal Article

Reduced metastasis-suppressor gene mRNA-expression in breast cancer brain metastases.

Journal of cancer research and clinical oncology ·Vol. 131 ·No. 3 ·2005-03-00 ·Pages 191-8

Stark AM, Tongers K, Maass N, Mehdorn HM, Held-Feindt J

Abstract

Brain metastases are an increasingly common complication in breast cancer patients. The Metastasis Suppressor Genes (MSG) Nm23, KISS1, KAI1, BRMS1, and Mkk4 have been associated with the metastatic potential of breast cancer in vitro and in vivo. The mRNA expression of Nm23, KISS1, KAI1, BRMS1, and Mkk4 in fresh frozen tissue samples of brain metastases from ductal invasive breast cancer specimens was examined in relation to primary tumors. In a first step, mRNA expression screening was carried out using a semi-quantitative RT-PCR approach, in a second step quantitative real-time RT-PCR was performed on selected specimens. By immunohistochemical staining, gene products were visualized on the protein level. Semi-quantitative RT-PCR revealed reduced mRNA expression of Nm23, KISS1, KAI1, BRMS, and Mkk4 in brain metastases. Results for KISS1, KAI1, BRMS, and Mkk4 were confirmed by real-time RT-PCR. In detail, mRNA expression reduction in breast cancer brain metastases was tenfold. Expression of MSG could be confirmed by immunohistochemical staining on protein level. Our investigations revealed significantly reduced mRNA expression of metastases suppressor genes KISS1, KAI1, BRMS1, and Mkk4 in breast cancer brain metastasis. Particularly, in the case of KISS1 and Mkk4, an important role for future treatment of patients with breast cancer brain metastatic lesions can be assumed.

MeSH Terms
Antigens, CD/analysis Biomarkers, Tumor/analysis,genetics Brain Neoplasms/chemistry,genetics,secondary Breast Neoplasms/chemistry,genetics,pathology Female Gene Expression Regulation, Neoplastic Genes, Tumor Suppressor Humans Immunohistochemistry Kangai-1 Protein Kisspeptins MAP Kinase Kinase 4/analysis Membrane Glycoproteins/analysis NM23 Nucleoside Diphosphate Kinases Neoplasm Proteins/analysis Nucleoside-Diphosphate Kinase/analysis Proteins/analysis Proto-Oncogene Proteins/analysis RNA, Messenger/analysis Repressor Proteins Reverse Transcriptase Polymerase Chain Reaction Tumor Suppressor Proteins/analysis,genetics
Chemicals
Antigens, CD BRMS1 protein, human Biomarkers, Tumor CD82 protein, human KISS1 protein, human Kangai-1 Protein Kisspeptins Membrane Glycoproteins NM23 Nucleoside Diphosphate Kinases Neoplasm Proteins Proteins Proto-Oncogene Proteins RNA, Messenger Repressor Proteins Tumor Suppressor Proteins MAP Kinase Kinase 4 NME1 protein, human Nucleoside-Diphosphate Kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Stark Andreas M
Department of Neurosurgery, University of Schleswig-Holstein Medical Center, Campus Kiel, Schittenhelmstrasse 10, 24105 Kiel, Germany.
Tongers Kerrin
Maass Nicolai
Mehdorn H Maximilian
Held-Feindt Janka
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Article Info
Journal
Journal of cancer research and clinical oncology
Abbr.
J Cancer Res Clin Oncol
ISSN
0171-5216
DOI
10.1007/s00432-004-0629-9
Published
2005-03-00
Epub
2004-00-08
Pages
191-8
Language
English
Region
Germany
NLM ID
7902060
Subset
IM
External Links
PubMed source DOI: 10.1007/s00432-004-0629-9 View journal details
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