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PMID: 12547718 Published · ppublish English Journal Article

Tumor suppressor role of KiSS-1 in bladder cancer: loss of KiSS-1 expression is associated with bladder cancer progression and clinical outcome.

The American journal of pathology ·Vol. 162 ·No. 2 ·2003-02-00 ·Pages 609-17

Sanchez-Carbayo M, Capodieci P, Cordon-Cardo C

Abstract

The expression profiles of nine bladder cancer cell lines were compared against a pool containing equal total RNA quantities of each of them. Lower expression of KiSS-1 was revealed in cells derived from the most advanced bladder tumors. When comparing 15 primary bladder tumors versus a pool of four bladder cancer cell lines, lower transcript levels of KiSS-1 were observed in the invasive bladder carcinomas as compared to superficial tumors. KiSS-1 expression ratios provided prognostic information. The expression pattern of KiSS-1 transcripts was analyzed using in situ hybridization in nine bladder cancer cells, paired normal urothelium and bladder tumor samples (n = 25), and tissue microarrays of bladder tumors (n = 173). We observed complete loss of KiSS-1 in all invasive tumors under study as compared to their respective normal urothelium. The expression of KiSS-1 was found to be significantly associated with histopathological stage. Patients with lower KiSS-1 expression showed a direct correlation with overall survival in a subset of bladder tumors whose follow-up was available (n = 69). We did not observe any significant differential KiSS-1 expression along cell cycle by sorting analysis. A potential tumor suppressor role in bladder cancer was revealed for KiSS-1. Moreover, it showed predictive value by identifying patients with poor outcome.

MeSH Terms
Adult Aged Aged, 80 and over Carcinoma in Situ/genetics,mortality,pathology Disease Progression Female Genes, Retinoblastoma Genes, Tumor Suppressor Genes, p53 Humans In Situ Hybridization Kisspeptins Male Middle Aged Neoplasm Staging Oligonucleotide Array Sequence Analysis Proteins/genetics Smoking Survival Analysis Time Factors Treatment Outcome Tumor Cells, Cultured Tumor Suppressor Proteins Urinary Bladder Neoplasms/genetics,mortality,pathology
Chemicals
KISS1 protein, human Kisspeptins Proteins Tumor Suppressor Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sanchez-Carbayo Marta
Division of Molecular Pathology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA. sanchezm@mskcc.org
Capodieci Paola
Cordon-Cardo Carlos
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
DOI
10.1016/S0002-9440(10)63854-0
Email
sanchezm@mskcc.org
Published
2003-02-00
Pages
609-17
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1851149
Subset
IM
External Links
PubMed source DOI: 10.1016/S0002-9440(10)63854-0 PMC Full Text View journal details
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