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PMID: 17475768 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Accumulation of polyadenylated mRNA, Pab1p, eIF4E, and eIF4G with P-bodies in Saccharomyces cerevisiae.

Molecular biology of the cell ·Vol. 18 ·No. 7 ·2007-07-00 ·Pages 2592-602

Brengues M, Parker R

Abstract

Recent experiments have shown that mRNAs can move between polysomes and P-bodies, which are aggregates of nontranslating mRNAs associated with translational repressors and the mRNA decapping machinery. The transitions between polysomes and P-bodies and how the poly(A) tail and the associated poly(A) binding protein 1 (Pab1p) may affect this process are unknown. Herein, we provide evidence that poly(A)(+) mRNAs can enter P-bodies in yeast. First, we show that both poly(A)(-) and poly(A)(+) mRNA become translationally repressed during glucose deprivation, where mRNAs accumulate in P-bodies. In addition, both poly(A)(+) transcripts and/or Pab1p can be detected in P-bodies during glucose deprivation and in stationary phase. Cells lacking Pab1p have enlarged P-bodies, suggesting that Pab1p plays a direct or indirect role in shifting the equilibrium of mRNAs away from P-bodies and into translation, perhaps by aiding in the assembly of a type of mRNP within P-bodies that is poised to reenter translation. Consistent with this latter possibility, we observed the translation initiation factors (eIF)4E and eIF4G in P-bodies at a low level during glucose deprivation and at high levels in stationary phase. Moreover, Pab1p exited P-bodies much faster than Dcp2p when stationary phase cells were given fresh nutrients. Together, these results suggest that polyadenylated mRNAs can enter P-bodies, and an mRNP complex including poly(A)(+) mRNA, Pab1p, eIF4E, and eIF4G2 may represent a transition state during the process of mRNAs exchanging between P-bodies and translation.

MeSH Terms
Cell Nucleus Structures/metabolism Endoribonucleases Eukaryotic Initiation Factor-4E/metabolism Eukaryotic Initiation Factor-4G/metabolism Glucose/deficiency Poly(A)-Binding Proteins/metabolism Protein Biosynthesis RNA Transport RNA, Messenger/metabolism Saccharomyces cerevisiae/cytology,metabolism Saccharomyces cerevisiae Proteins/metabolism
Chemicals
Eukaryotic Initiation Factor-4E Eukaryotic Initiation Factor-4G Poly(A)-Binding Proteins RNA, Messenger Saccharomyces cerevisiae Proteins pab1 protein, S cerevisiae DCP2 protein, S cerevisiae Endoribonucleases Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brengues Muriel
Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721-0106, USA.
Parker Roy
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e06-12-1149
Published
2007-07-00
Epub
2007-00-02
Pages
2592-602
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1924816
Subset
IM
Grants
NIGMS NIH HHS · R01 GM045443 · United States
NIGMS NIH HHS · R37 GM045443 · United States
NIGMS NIH HHS · GM-45443 · United States
External Links
PubMed source DOI: 10.1091/mbc.e06-12-1149 PMC Full Text View journal details
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