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PMID: 16777600 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Targeting of aberrant mRNAs to cytoplasmic processing bodies.

Cell ·Vol. 125 ·No. 6 ·2006-06-16 ·Pages 1095-109

Sheth U, Parker R

Abstract

In eukaryotes, a specialized pathway of mRNA degradation termed nonsense-mediated decay (NMD) functions in mRNA quality control by recognizing and degrading mRNAs with aberrant termination codons. We demonstrate that NMD in yeast targets premature termination codon (PTC)-containing mRNA to P-bodies. Upf1p is sufficient for targeting mRNAs to P-bodies, whereas Upf2p and Upf3p act, at least in part, downstream of P-body targeting to trigger decapping. The ATPase activity of Upf1p is required for NMD after the targeting of mRNAs to P-bodies. Moreover, Upf1p can target normal mRNAs to P-bodies but not promote their degradation. These observations lead us to propose a new model for NMD wherein two successive steps are used to distinguish normal and aberrant mRNAs.

MeSH Terms
Adaptor Proteins, Signal Transducing Codon, Nonsense Cytoplasmic Structures/metabolism Models, Biological RNA Helicases/genetics,metabolism RNA Stability RNA Transport RNA, Fungal/metabolism RNA, Messenger/metabolism RNA-Binding Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Trans-Activators/genetics,metabolism
Chemicals
Adaptor Proteins, Signal Transducing Codon, Nonsense NMD2 protein, S cerevisiae RNA, Fungal RNA, Messenger RNA-Binding Proteins Saccharomyces cerevisiae Proteins Trans-Activators UPF3 protein, S cerevisiae NAM7 protein, S cerevisiae RNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sheth Ujwal
Department of Molecular and Cellular Biology, University of Arizona, Tucson, 85721, USA.
Parker Roy
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/j.cell.2006.04.037
Published
2006-06-16
Pages
1095-109
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC1858659
Subset
IM
Grants
NIGMS NIH HHS · R01 GM045443 · United States
NIGMS NIH HHS · R37 GM045443 · United States
NIGMS NIH HHS · GM45443 · United States
Corrections
CommentIn
PMID: 16777595
External Links
PubMed source DOI: 10.1016/j.cell.2006.04.037 PMC Full Text View journal details
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