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PMID: 17381149 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Imaging mass spectrometry of neuropeptides in decapod crustacean neuronal tissues.

Journal of proteome research ·Vol. 6 ·No. 5 ·2007-05-00 ·Pages 1782-91

DeKeyser SS, Kutz-Naber KK, Schmidt JJ, Barrett-Wilt GA, Li L

Abstract

Imaging mass spectrometry (IMS) of neuropeptides in crustacean neuronal tissues was performed on a MALDI-TOF/TOF instrument. Sample preparation protocols were developed for the sensitive detection of these highly complex endogenous signaling molecules. The neuromodulatory complements of the pericardial organ (PO) and brain of the Jonah crab, Cancer borealis, were mapped. Distributions of peptide isoforms belonging to 10 neuropeptide families were investigated using the IMS technique. Often, neuropeptides of high sequence homology were similarly located. However, two RFamide-family peptides and a truncated orcokinin peptide were mapped to locations distinct from other members of their respective families. Over 30 previously sequenced neuropeptides were identified based on mass measurement. For increased confidence of identification, select peptides were fragmented by post-source decay (PSD) and collisional-induced dissociation (CID). Collectively, this organ-level IMS study elucidates the spatial relationships between multiple neuropeptide isoforms of the same family as well as the relative distributions of neuropeptide families.

MeSH Terms
Amino Acid Sequence Animals Brachyura/anatomy & histology,chemistry Mass Spectrometry/methods Molecular Sequence Data Nerve Tissue/chemistry Nerve Tissue Proteins/chemistry,genetics,metabolism Neuropeptides/chemistry,genetics,metabolism Protein Isoforms/chemistry,genetics,metabolism
Chemicals
Nerve Tissue Proteins Neuropeptides Protein Isoforms
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
DeKeyser Stephanie S
School of Pharmacy and Department of Chemistry, University of Wisconsin-Madison, 777 Highland Avenue, Madison, Wisconsin 53705-2222, USA.
Kutz-Naber Kimberly K
Schmidt Joshua J
Barrett-Wilt Gregory A
Li Lingjun
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Article Info
Journal
Journal of proteome research
Abbr.
J Proteome Res
ISSN
1535-3893
DOI
10.1021/pr060603v
Published
2007-05-00
Epub
2007-00-24
Pages
1782-91
Language
English
Region
United States
NLM ID
101128775
PMCID
PMC2529365
Subset
IM
Grants
NIGMS NIH HHS · T32 GM008505 · United States
NIDDK NIH HHS · R01 DK071801-01A1 · United States
NIDDK NIH HHS · R01 DK071801-02S1 · United States
NIDDK NIH HHS · R01 DK071801-02 · United States
NIDDK NIH HHS · R01 DK071801 · United States
NIDDK NIH HHS · 1R01DK071801 · United States
External Links
PubMed source DOI: 10.1021/pr060603v PMC Full Text View journal details
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