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PMID: 17055739 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Imaging of peptides in the rat brain using MALDI-FTICR mass spectrometry.

Journal of the American Society for Mass Spectrometry ·Vol. 18 ·No. 1 ·2007-01-00 ·Pages 145-51

Taban IM, Altelaar AF, van der Burgt YE, McDonnell LA, Heeren RM, Fuchser J, Baykut G

Abstract

Analytical methods are pursued to measure the identity and location of biomolecules down to the subcellular (microm) level. Available mass spectrometric imaging methods either compromise localization accuracy or identification accuracy in their analysis of surface biomolecules. In this study, imaging FTICR-MS is applied for the spatially resolved mass analysis of rat brain tissue with the aim to optimize protein identification by the high mass accuracy and online MS/MS capabilities of the technique. Mass accuracies up to 6 ppm were obtained in the direct MALDI-analysis of the tissue together with a spatial resolution of 200 microm. The spatial distributions of biomolecules differing in mass by less than 0.1 Da could be resolved, and are shown to differ significantly. Online MS/MS analysis of selected ions was demonstrated. A comparison of the FTICR-MS imaging results with stigmatic TOF imaging on the same sample is presented. To reduce the extended measuring times involved, it is recommended to restrict the FTICR-MS analyses to areas of interest as can be preselected by other, faster imaging methods.

MeSH Terms
Animals Brain Chemistry Image Processing, Computer-Assisted/methods Male Peptides/analysis,chemistry Rats Rats, Wistar Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/instrumentation,methods Spectroscopy, Fourier Transform Infrared/instrumentation,methods Tandem Mass Spectrometry
Chemicals
Peptides
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Taban Ioana M
FOM Institute for Atomic and Molecular Physics, Amsterdam, The Netherlands.
Altelaar A F Maarten
van der Burgt Yuri E M
McDonnell Liam A
Heeren Ron M A
Fuchser Jens
Baykut Gökhan
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Article Info
Journal
Journal of the American Society for Mass Spectrometry
Abbr.
J Am Soc Mass Spectrom
ISSN
1044-0305
DOI
10.1016/j.jasms.2006.09.017
Published
2007-01-00
Epub
2006-00-19
Pages
145-51
Language
English
Region
United States
NLM ID
9010412
Subset
IM
External Links
PubMed source DOI: 10.1016/j.jasms.2006.09.017 View journal details
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