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PMID: 17364015 Published · ppublish English Journal Article

Analysis of Mammalian Cell Cytoplasm with Electrophoresis in Nanometer Inner Diameter Capillaries.

Electroanalysis ·Vol. 17 ·No. 13 ·2005-07-00 ·Pages 1192-1197

Woods LA, Powell PR, Paxon TL, Ewing AG

Abstract

Capillary electrophoresis in 770 nanometer inner diameter capillaries coupled to electrochemical detection with an etched electrode matching an etched capillary (etched electrochemical detection) has been used with ultrasmall sampling to inject subcellular samples from intact single mammalian cells. Separations of cytoplasmic samples taken from rat pheochromocytoma cells have been achieved. As little as 8% of the total volume of a single cell has been sampled and analyzed. Dopamine has been identified and quantified in these PC12 cells using this technique. The average cytoplasmic level of dopamine in rat pheochromocytoma cells has been determined to be 240 ± 60 μM. The use of electrophoresis in 770 nanometer inner diameter capillaries with electrochemical detection to monitor cytoplasmic neurotransmitters at the single cell level can provide information about complex cellular functions such as neurotransmitter storage and synthesis.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Woods Lori A
Department of Chemistry, 104 Chemistry Building, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Powell Paula R
Paxon Tracy L
Ewing Andrew G
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Article Info
Journal
Electroanalysis
Abbr.
Electroanalysis
ISSN
1040-0397
Published
2005-07-00
Pages
1192-1197
Language
English
Region
Germany
NLM ID
8908702
PMCID
PMC1821352
Grants
NIBIB NIH HHS · R01 EB000352-05 · United States
NIBIB NIH HHS · R01 EB002016 · United States
NIBIB NIH HHS · R01 EB002016-10 · United States
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