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PMID: 10884311 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

VMAT-Mediated changes in quantal size and vesicular volume.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 20 ·No. 14 ·2000-07-15 ·Pages 5276-82

Colliver TL, Pyott SJ, Achalabun M, Ewing AG

Abstract

It has been well established that the volume of secretory vesicles can be modulated. However, we present the first data demonstrating that the amount of transmitter in a vesicle can regulate its volume. Amperometry and transmission electron microscopy have been used to determine that l-3,4-dihydroxyphenylalanine and reserpine increase and decrease, respectively, the volume of single pheochromocytoma cell vesicles as well as their catecholamine content. Because changes in vesicular catecholamine content are tracked by changes in vesicle volume, our results indicate that when quantal size is altered via the vesicular monoamine transporter the concentration of catecholamines within the vesicles remains relatively constant. This previously unidentified cellular response provides new insight into how catecholamines can be packaged in and released from secretory vesicles.

MeSH Terms
Action Potentials/drug effects Adrenergic Uptake Inhibitors/pharmacology Animals Catecholamines/biosynthesis,metabolism Cell Size/drug effects Dihydroxyphenylalanine/pharmacology Dopamine Agents/pharmacology Electrochemistry Membrane Glycoproteins/metabolism Membrane Transport Proteins Microscopy, Electron Neuropeptides Organelles/drug effects,metabolism,ultrastructure PC12 Cells Rats Reserpine/pharmacology Vesicular Biogenic Amine Transport Proteins Vesicular Monoamine Transport Proteins
Chemicals
Adrenergic Uptake Inhibitors Catecholamines Dopamine Agents Membrane Glycoproteins Membrane Transport Proteins Neuropeptides Vesicular Biogenic Amine Transport Proteins Vesicular Monoamine Transport Proteins Dihydroxyphenylalanine Reserpine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Colliver T L
Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Pyott S J
Achalabun M
Ewing A G
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
2000-07-15
Pages
5276-82
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6772308
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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