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PMID: 9396001 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Review

Quantitative measurements of released amines from individual exocytosis events.

Molecular neurobiology ·Vol. 15 ·No. 1 ·1997-08-00 ·Pages 1-16

Clark RA, Ewing AG

Abstract

Chemical analysis of single cells is an area of great interest in the biological sciences. Single-cell systems are being utilized as a model to understand in vivo processes better. One method that is moving to the forefront in cellular analysis is electrochemistry. Owing to their rapid response time and small dimensions, voltammetric microelectrode techniques, such as amperometry and fast-scan voltammetry, have made it possible to monitor minute amounts of biological compounds and transiently occurring chemical events in cellular systems. The application of these methods to the quantitation of individual vesicular release events from single cells is overviewed here. The application of electrochemical monitoring to several types of cultured cells, including bovine adrenal chromaffin cells, rat pheochromocytoma (PC12) cells, beige mouse mast cells, superior cervical ganglion neurons, and human pancreatic beta-cells, as well as to the invertebrate systems, the leech Hirudo medicinalis, and pond snail Planorbis corneus has provided a wealth of new information concerning exocytosis. Results obtained from the studies highlight the potential of electrochemical techniques in cellular analysis to contribute to our understanding of molecular and pharmacological effects on exocytosis. This article overviews work done on all the above cell types with an emphasis on PC12 cells.

MeSH Terms
Adrenal Gland Neoplasms Adrenal Medulla/physiology Animals Catecholamines/analysis,metabolism Cattle Cells, Cultured Chromaffin Cells/physiology Electrochemistry/methods Exocytosis Humans Islets of Langerhans/physiology Mast Cells/physiology Mice Mice, Mutant Strains Neurons/physiology PC12 Cells Pheochromocytoma Rats Superior Cervical Ganglion/physiology
Chemicals
Catecholamines
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clark R A
Department of Chemistry, Pennsylvania State University, University Park 16802, USA.
Ewing A G
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Article Info
Journal
Molecular neurobiology
Abbr.
Mol Neurobiol
ISSN
0893-7648
DOI
10.1007/BF02740612
Published
1997-08-00
Pages
1-16
Language
English
Region
United States
NLM ID
8900963
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02740612 View journal details
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