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PMID: 7708668 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Colocalization of calcium entry and exocytotic release sites in adrenal chromaffin cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 7 ·1995-03-28 ·Pages 2474-8

Robinson IM, Finnegan JM, Monck JR, Wightman RM, Fernandez JM

Abstract

"Snapshot" images of localized Ca2+ influx into patch-clamped chromaffin cells were captured by using a recently developed pulsed-laser imaging system. Transient opening of voltage-sensitive Ca2+ channels gave rise to localized elevations of Ca2+ that had the appearance of either "hotspots" or partial rings found immediately beneath the plasma membrane. When the Ca2+ imaging technique was employed in conjunction with flame-etched carbon-fiber electrodes to spatially map the release sites of catecholamines, it was observed that the sites of Ca2+ entry and catecholamine release were colocalized. These results provide functional support for the idea that secretion occurs from "active zone"-like structures in neuroendocrine cells.

MeSH Terms
Adrenal Medulla/metabolism Animals Biological Transport Calcium/metabolism Catecholamines/metabolism Cattle Cell Membrane/metabolism,ultrastructure Cells, Cultured Electrophysiology/methods Exocytosis Spectrometry, Fluorescence
Chemicals
Catecholamines Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Robinson I M
Department of Physiology and Biophysics, Mayo Clinic, Rochester, MN 55905, USA.
Finnegan J M
Monck J R
Wightman R M
Fernandez J M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.7.2474
Published
1995-03-28
Pages
2474-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC42240
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.92.7.2474 PMC Full Text View journal details
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