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PMID: 1732213 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning and DNA sequence of a mycoplasmal recA gene.

Journal of bacteriology ·Vol. 174 ·No. 3 ·1992-02-00 ·Pages 778-84

Dybvig K, Woodard A

Abstract

Mycoplasmas are wall-less prokaryotes phylogenetically related to gram-positive bacteria. In order to investigate DNA recombination in these organisms, we have cloned the recA gene from the mycoplasma Acholeplasma laidlawii. DNA sequence data indicate extensive homology between the A. laidlawii recA gene and recA genes from other bacteria, particularly Bacillus subtilis. The recA sequences from three A. laidlawii strains (strains JA1, K2, and 8195) were compared, and surprisingly, the gene from A. laidlawii 8195 was found to contain a nonsense mutation that results in truncation of 36 amino acids from the carboxyl terminus of the RecA protein. By using sensitivity to UV irradiation as a measure of DNA repair, strain 8195 had an apparent RecA- phenotype. When carried on a multicopy plasmid, the wild-type A. laidlawii recA gene was detrimental to growth of Escherichia coli, perhaps because of improper regulation of the RecA protein.

MeSH Terms
Acholeplasma laidlawii/genetics Amino Acid Sequence Base Sequence Cloning, Molecular Escherichia coli/genetics Genetic Complementation Test Molecular Sequence Data Mutagenesis Rec A Recombinases/classification,genetics Sequence Homology, Nucleic Acid Ultraviolet Rays/adverse effects
Chemicals
Rec A Recombinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dybvig K
Department of Comparative Medicine, University of Alabama, Birmingham 35294.
Woodard A
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38 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.3.778-784.1992
Published
1992-02-00
Pages
778-84
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206154
Subset
IM
Grants
NIAID NIH HHS · AI25640 · United States
NIAID NIH HHS · AI31144 · United States
Databases
GENBANK
L01135, L01136, L01137, L01138, L01139, L01140, L01141, M81465, M98391, Z11768
External Links
PubMed source DOI: 10.1128/jb.174.3.778-784.1992 PMC Full Text View journal details
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