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PMID: 202858 Published · ppublish English Journal Article

Deletion mutants of temperate Bacillus subtilis bacteriophage phi105.

Molecular & general genetics : MGG ·Vol. 155 ·No. 3 ·1977-10-24 ·Pages 241-7

Flock JI

Abstract

Six deletion mutants of temperate Bacillus subtilis phage phi105 have been isolated on the basis of their increased resistance to chelating agents. The size and position of the deletions was determined by electronmicroscopy of DNA heteroduplexes. All deletions are located in a region about 55-70% from one end of the DNA molecule, in the right half of the known genetic map of the phage. The segment 55-65% does not contain any genes essential for lytic growth or lysogenization. A gene(s) for immunity is located in a segment 65-70% from the left end. By electronmicroscopy by partially denatured phi105 DNA two A-T rich regions have been localized in the right half of the molecule. One of these regions falls within the non-essential 55-65% DNA segment.

MeSH Terms
Bacillus subtilis Bacteriophages/drug effects,genetics,metabolism Chelating Agents/pharmacology Chromosome Deletion DNA, Viral/metabolism Diphosphates/pharmacology Drug Resistance Lysogeny Mutation Nucleic Acid Hybridization Transfection
Chemicals
Chelating Agents DNA, Viral Diphosphates
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Flock J I
References (20)
20 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00272803
Published
1977-10-24
Pages
241-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00272803
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