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PMID: 2684784 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Escherichia coli recA deletion strains that are highly competent for transformation and for in vivo phage packaging.

Gene ·Vol. 82 ·No. 2 ·1989-10-30 ·Pages 313-5

Kurnit DM

Abstract

I describe the construction of a variety of Escherichia coli recA deletion strains designed to facilitate molecular cloning. These recA deletion strains permit the efficient cloning of foreign inserts carried in plasmid, phage, cosmid, phasmid (phage-plasmid hybrid) or phosmid (phage-cosmid hybrid) vectors.

MeSH Terms
Cloning, Molecular/methods Escherichia coli/genetics Genetic Vectors Rec A Recombinases/genetics Transformation, Bacterial
Chemicals
Rec A Recombinases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kurnit D M
Howard Hughes Medical Institute, Department of Pediatrics, University of Michigan Medical Center, Ann Arbor 48109-0650.
Article Info
Journal
Gene
Abbr.
Gene
ISSN
0378-1119
DOI
10.1016/0378-1119(89)90056-5
Published
1989-10-30
Pages
313-5
Language
English
Region
Netherlands
NLM ID
7706761
Subset
IM
Grants
NICHD NIH HHS · HD20118 · United States
External Links
PubMed source DOI: 10.1016/0378-1119(89)90056-5 View journal details
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