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PMID: 17229886 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Avl9p, a member of a novel protein superfamily, functions in the late secretory pathway.

Molecular biology of the cell ·Vol. 18 ·No. 4 ·2007-04-00 ·Pages 1203-19

Harsay E, Schekman R

Abstract

The branching of exocytic transport routes in both yeast and mammalian cells has complicated studies of the late secretory pathway, and the mechanisms involved in exocytic cargo sorting and exit from the Golgi and endosomes are not well understood. Because cargo can be sorted away from a blocked route and secreted by an alternate route, mutants defective in only one route do not exhibit a strong secretory phenotype and are therefore difficult to isolate. In a genetic screen designed to isolate such mutants, we identified a novel conserved protein, Avl9p, the absence of which conferred lethality in a vps1Delta apl2Delta strain background (lacking a dynamin and an adaptor-protein complex 1 subunit). Depletion of Avl9p in this strain resulted in secretory defects as well as accumulation of Golgi-like membranes. The triple mutant also had a depolarized actin cytoskeleton and defects in polarized secretion. Overexpression of Avl9p in wild-type cells resulted in vesicle accumulation and a post-Golgi defect in secretion. Phylogenetic analysis indicated evolutionary relationships between Avl9p and regulators of membrane traffic and actin function.

MeSH Terms
Actins/metabolism Amino Acid Sequence Cell Polarity/genetics Conserved Sequence Exocytosis Gene Expression Regulation, Fungal Golgi Apparatus/metabolism,ultrastructure Molecular Biology/methods Mutation Phylogeny Saccharomyces cerevisiae/physiology Saccharomyces cerevisiae Proteins/genetics,metabolism Secretory Vesicles/genetics,metabolism Vesicular Transport Proteins/genetics,metabolism
Chemicals
Actins Avl9 protein, S cerevisiae Saccharomyces cerevisiae Proteins Vesicular Transport Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Harsay Edina
Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA. harsay@ku.edu
Schekman Randy
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e06-11-1035
Email
harsay@ku.edu
Published
2007-04-00
Epub
2007-00-17
Pages
1203-19
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1838974
Subset
IM
Grants
NCRR NIH HHS · P20 RR015563 · United States
NIGMS NIH HHS · R01 GM026755 · United States
NCRR NIH HHS · P20 RR15563-06 · United States
NIGMS NIH HHS · R01 GM26755 · United States
External Links
PubMed source DOI: 10.1091/mbc.e06-11-1035 PMC Full Text View journal details
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