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PMID: 11239398 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Protein kinase D regulates the fission of cell surface destined transport carriers from the trans-Golgi network.

Cell ·Vol. 104 ·No. 3 ·2001-02-09 ·Pages 409-20

Liljedahl M, Maeda Y, Colanzi A, Ayala I, Van Lint J, Malhotra V

Abstract

When a kinase inactive form of Protein Kinase D (PKD-K618N) was expressed in HeLa cells, it localized to the trans-Golgi network (TGN) and caused extensive tubulation. Cargo that was destined for the plasma membrane was found in PKD-K618N-containing tubes but the tubes did not detach from the TGN. As a result, the transfer of cargo from TGN to the plasma membrane was inhibited. We have also demonstrated the formation and subsequent detachment of cargo-containing tubes from the TGN in cells stably expressing low levels of PKD-K618N. Our results suggest that PKD regulates the fission from the TGN of transport carriers that are en route to the cell surface.

MeSH Terms

Biological Transport CD4 Antigens/metabolism Cell Line Cell Membrane/enzymology,metabolism Clathrin/metabolism Coatomer Protein/metabolism Dose-Response Relationship, Drug Endoplasmic Reticulum/metabolism Endosomes/metabolism Fluorescent Antibody Technique Glutathione Transferase/metabolism Glycoproteins HeLa Cells Humans Membrane Glycoproteins/metabolism Membrane Proteins Phosphorylation Precipitin Tests Protein Kinase C/genetics,physiology Protein Transport Recombinant Proteins/metabolism Temperature Time Factors Transfection Viral Envelope Proteins/metabolism trans-Golgi Network/metabolism

Chemicals

CD4 Antigens Clathrin Coatomer Protein G protein, vesicular stomatitis virus Glycoproteins Membrane Glycoproteins Membrane Proteins Recombinant Proteins TGOLN2 protein, human Viral Envelope Proteins Glutathione Transferase protein kinase D Protein Kinase C

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Liljedahl M

Biology Department, University of California, San Diego, La Jolla, CA 92093, USA.

Maeda Y

Colanzi A

Ayala I

Van Lint J

Malhotra V

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/s0092-8674(01)00228-8

Published

2001-02-09

Pages

409-20

Language

English

Region

United States

NLM ID

0413066

Subset

External Links

PubMed source DOI: 10.1016/s0092-8674(01)00228-8 View journal details

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Protein kinase D regulates the fission of cell surface destined transport carriers from the trans-Golgi network.

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