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PMID: 16855022 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Chs5/6 complex: a multiprotein complex that interacts with and conveys chitin synthase III from the trans-Golgi network to the cell surface.

Molecular biology of the cell ·Vol. 17 ·No. 10 ·2006-10-00 ·Pages 4157-66

Sanchatjate S, Schekman R

Abstract

In Saccharomyces cerevisiae, the polysaccharide chitin is deposited at the mother bud junction by an integral membrane enzyme, chitin synthase 3 (Chs3p). The traffic of Chs3p to the cell surface from the trans-Golgi network (TGN) depends on two proteins, Chs5p and Chs6p, which sort selected cargo proteins into secretory vesicles. We have found that Chs5p forms a large higher-order complex of around 1 MDa with Chs6p and three Chs6 paralogs: Bch1p, Bud7p, and Bch2p. The Chs5/6 complex transiently interacts with its cargo, Chs3p, and the presence of Chs3p in the complex is dependent on every subunit. Chs5p and Chs6p have unique and crucial roles in Chs3p transport because either a chs5delta or chs6delta mutant drastically reduces the level of Chs3p bound to the remaining subunits of the complex. Bch1p and Bud7p appear to have a redundant function in Chs3p transport because deletion of both is necessary to displace Chs3p from the complex. The role of Bch2p in Chs3p binding is the least important. Chs5p is essential for structural integrity of the Chs5/6 complex and may act as a scaffold through which the other subunits assemble. Our results suggest a model of protein sorting at the TGN that involves a peripheral, possibly coat, complex that includes multiple related copies of a specificity determining subunit.

MeSH Terms
Adaptor Proteins, Vesicular Transport Biological Transport Carrier Proteins/metabolism Cell Membrane/metabolism Chitin Synthase/metabolism,physiology Fungal Proteins/metabolism,physiology Gene Expression Regulation, Fungal Multiprotein Complexes/metabolism Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins/metabolism trans-Golgi Network/metabolism
Chemicals
Adaptor Proteins, Vesicular Transport CHS6 protein, S cerevisiae Carrier Proteins Fungal Proteins Multiprotein Complexes Saccharomyces cerevisiae Proteins Chitin Synthase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sanchatjate Siraprapha
Department of Molecular and Cell Biology, Howard Hughes Medical Institute, Barker Hall, University of California, Berkeley, Berkeley, CA 94720, USA.
Schekman Randy
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e06-03-0210
Published
2006-10-00
Epub
2006-00-19
Pages
4157-66
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1635352
Subset
IM
Grants
NIGMS NIH HHS · R01 GM026755 · United States
NIGMS NIH HHS · GM26755 · United States
External Links
PubMed source DOI: 10.1091/mbc.e06-03-0210 PMC Full Text View journal details
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