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PMID: 1722078 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A DNA sequence upstream of the tet(O) gene is required for full expression of tetracycline resistance.

Antimicrobial agents and chemotherapy ·Vol. 35 ·No. 10 ·1991-10-00 ·Pages 2020-5

Wang Y, Taylor DE

Abstract

The DNA sequences upstream of the tet(O) and tet(M) open reading frames (ORFs) (ca. 300 bp) were found to share a higher degree of homology than those of the tet(O) and tet(M) ORFs themselves. A transcription initiation site for tet(O) was located by primer extension analysis. Campylobacter coli was found to use a promoter sequence different from that used by Escherichia coli. The sequence upstream of tet(O) was shown to be required in cis for high-level resistance to tetracycline.

Related Genes
tet(M) tet(O)
MeSH Terms
Base Sequence Campylobacter coli/genetics DNA, Bacterial/genetics Escherichia coli/genetics Gene Expression Regulation, Bacterial Microbial Sensitivity Tests Molecular Sequence Data Mutagenesis, Site-Directed Open Reading Frames RNA, Bacterial/genetics Sequence Homology, Nucleic Acid Tetracycline Resistance/genetics Transcription, Genetic
Chemicals
DNA, Bacterial RNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang Y
Department of Microbiology, University of Alberta, Edmonton, Canada.
Taylor D E
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25 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.35.10.2020
Published
1991-10-00
Pages
2020-5
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC245318
Subset
IM
Databases
GENBANK
M58727, M74450, M74451, M77181, S55854, S55874, S62061, S62063, S62066, S62712, S68604, S72596
External Links
PubMed source DOI: 10.1128/AAC.35.10.2020 PMC Full Text View journal details
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