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PMID: 17189430 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genome-wide redistribution of meiotic double-strand breaks in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 27 ·No. 5 ·2007-03-00 ·Pages 1868-80

Robine N, Uematsu N, Amiot F, Gidrol X, Barillot E, Nicolas A, Borde V

Abstract

Meiotic recombination is initiated by the formation of programmed DNA double-strand breaks (DSBs) catalyzed by the Spo11 protein. DSBs are not randomly distributed along chromosomes. To better understand factors that control the distribution of DSBs in budding yeast, we have examined the genome-wide binding and cleavage properties of the Gal4 DNA binding domain (Gal4BD)-Spo11 fusion protein. We found that Gal4BD-Spo11 cleaves only a subset of its binding sites, indicating that the association of Spo11 with chromatin is not sufficient for DSB formation. In centromere-associated regions, the centromere itself prevents DSB cleavage by tethered Gal4BD-Spo11 since its displacement restores targeted DSB formation. In addition, we observed that new DSBs introduced by Gal4BD-Spo11 inhibit surrounding DSB formation over long distances (up to 60 kb), keeping constant the number of DSBs per chromosomal region. Together, these results demonstrate that the targeting of Spo11 to new chromosomal locations leads to both local stimulation and genome-wide redistribution of recombination initiation and that some chromosomal regions are inherently cold regardless of the presence of Spo11.

MeSH Terms
Binding Sites Chromatin Immunoprecipitation DNA Breaks, Double-Stranded DNA, Fungal/genetics DNA-Binding Proteins Endodeoxyribonucleases Esterases/genetics,metabolism Genome, Fungal Meiosis Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction Protein Binding Protein Structure, Tertiary Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins/genetics,metabolism Transcription Factors/genetics,metabolism
Chemicals
DNA, Fungal DNA-Binding Proteins GAL4 protein, S cerevisiae Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors Endodeoxyribonucleases Esterases meiotic recombination protein SPO11
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Robine Nicolas
Institut Curie, Recombinaison et Instabilité Génétique, Centre de Recherche, UMR7147 CNRS-Institut Curie-Université P. et M. Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France. valerie.borde@curie.fr
Uematsu Norio
Amiot Franck
Gidrol Xavier
Barillot Emmanuel
Nicolas Alain
Borde Valérie
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.02063-06
Email
valerie.borde@curie.fr
Published
2007-03-00
Epub
2006-00-22
Pages
1868-80
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1820458
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.02063-06 PMC Full Text View journal details
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