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PMID: 15654098 Published · ppublish English Journal Article

Infrequent co-conversion of markers flanking a meiotic recombination initiation site in Saccharomyces cerevisiae.

Genetics ·Vol. 169 ·No. 3 ·2005-03-00 ·Pages 1353-67

Jessop L, Allers T, Lichten M

Abstract

To study the mechanism of meiotic recombination in Saccharomyces cerevisiae, we examined recombination in an interval where the majority of events are initiated at a single hotspot for DNA double-strand breaks (DSBs), with little or no expected contribution by outside initiation events. This interval contained infrequently corrected palindromic markers 300 bp to the left and 600 bp to the right of the DSB hotspot. Conversion of single markers occurred frequently, while conversion of both markers occurred rarely, and many of the tetrads in which both markers converted were the products of multiple events. These data indicate that most meiotic recombination intermediates are asymmetrically positioned around the initiating DSB, with a short (<300 bp) tract of heteroduplex DNA (hDNA) to one side and hDNA on the other side frequently extending 600 bp or more. One consequence of this asymmetry is the preferential concentration of crossovers in the vicinity of the initiating DSB.

MeSH Terms
Base Pair Mismatch/genetics Crossing Over, Genetic/genetics DNA Damage/genetics DNA, Fungal/genetics Diploidy Electrophoresis, Gel, Pulsed-Field Fungal Proteins/genetics,isolation & purification Genetic Markers Meiosis/genetics Models, Genetic Recombination, Genetic Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal Fungal Proteins Genetic Markers
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jessop Lea
Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.
Allers Thorsten
Lichten Michael
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.104.036509
Published
2005-03-00
Epub
2005-00-16
Pages
1353-67
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1449552
Subset
IM
External Links
PubMed source DOI: 10.1534/genetics.104.036509 PMC Full Text View journal details
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