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PMID: 1717990 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 20 ·1991-10-15 ·Pages 8865-9

Koken MH, Reynolds P, Jaspers-Dekker I, Prakash L, Prakash S, Bootsma D, Hoeijmakers JH

Abstract

The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin-conjugating enzyme (E2) that is required for DNA repair, damage-induced mutagenesis, and sporulation. We have cloned the two human RAD6 homologs, designated HHR6A and HHR6B. The two 152-amino acid human proteins share 95% sequence identity with each other and approximately 70% and approximately 85% overall identity with the homologs from yeasts (S. cerevisiae and Schizosaccharomyces pombe) and Drosophila melanogaster, respectively. Neither of the human RAD6 homologs possess the acidic C-terminal sequence present in the S. cerevisiae RAD6 protein. Genetic complementation experiments reveal that HHR6A as well as HHR6B can carry out the DNA repair and mutagenesis functions of RAD6 in S. cerevisiae rad6 delta mutants.

Related Genes
RAD6
MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA Probes DNA Repair/genetics Drosophila/genetics Fungal Proteins/genetics Gene Library Genes, Fungal Humans Ligases/genetics Molecular Sequence Data Poly A/genetics,isolation & purification RNA/genetics,isolation & purification RNA, Messenger Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Schizosaccharomyces/enzymology,genetics Sequence Homology, Nucleic Acid Ubiquitin-Conjugating Enzymes
Chemicals
DNA Probes Fungal Proteins RNA, Messenger Saccharomyces cerevisiae Proteins Poly A RNA RAD6 protein, S cerevisiae UBE2A protein, human UBE2B protein, human Ubiquitin-Conjugating Enzymes Ligases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Koken M H
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Reynolds P
Jaspers-Dekker I
Prakash L
Prakash S
Bootsma D
Hoeijmakers J H
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.20.8865
Published
1991-10-15
Pages
8865-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52611
Subset
IM
Grants
NCI NIH HHS · CA41261 · United States
NIGMS NIH HHS · GM19261 · United States
Databases
GENBANK
M74524, M74525, M75995, M75996, M75997, M75998, M75999, M76000, S59486, S59488
External Links
PubMed source DOI: 10.1073/pnas.88.20.8865 PMC Full Text View journal details
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