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PMID: 17178837 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

RSC mobilizes nucleosomes to improve accessibility of repair machinery to the damaged chromatin.

Molecular and cellular biology ·Vol. 27 ·No. 5 ·2007-03-00 ·Pages 1602-13

Shim EY, Hong SJ, Oum JH, Yanez Y, Zhang Y, Lee SE

Abstract

Repair of DNA double-strand breaks (DSBs) protects cells and organisms, as well as their genome integrity. Since DSB repair occurs in the context of chromatin, chromatin must be modified to prevent it from inhibiting DSB repair. Evidence supports the role of histone modifications and ATP-dependent chromatin remodeling in repair and signaling of chromosome DSBs. The key questions are, then, what the nature of chromatin altered by DSBs is and how remodeling of chromatin facilitates DSB repair. Here we report a chromatin alteration caused by a single HO endonuclease-generated DSB at the Saccharomyces cerevisiae MAT locus. The break induces rapid nucleosome migration to form histone-free DNA of a few hundred base pairs immediately adjacent to the break. The DSB-induced nucleosome repositioning appears independent of end processing, since it still occurs when the 5'-to-3' degradation of the DNA end is markedly reduced. The tetracycline-controlled depletion of Sth1, the ATPase of RSC, or deletion of RSC2 severely reduces chromatin remodeling and loading of Mre11 and Yku proteins at the DSB. Depletion of Sth1 also reduces phosphorylation of H2A, processing, and joining of DSBs. We propose that RSC-mediated chromatin remodeling at the DSB prepares chromatin to allow repair machinery to access the break and is vital for efficient DSB repair.

MeSH Terms
Chromatin/genetics,metabolism Chromatin Immunoprecipitation Chromosomes, Fungal DNA Breaks, Double-Stranded DNA Repair DNA, Fungal DNA-Binding Proteins/metabolism Nucleosomes/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/metabolism Transcription Factors/metabolism
Chemicals
Chromatin DNA, Fungal DNA-Binding Proteins Nucleosomes RSC complex, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Shim Eun Yong
Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA.
Hong Soo Jin
Oum Ji-Hyun
Yanez Yvonne
Zhang Yu
Lee Sang Eun
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.01956-06
Published
2007-03-00
Epub
2006-00-18
Pages
1602-13
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1820475
Subset
IM
Grants
NIEHS NIH HHS · R01 ES012244 · United States
NIEHS NIH HHS · ES012244 · United States
External Links
PubMed source DOI: 10.1128/MCB.01956-06 PMC Full Text View journal details
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