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PMID: 1716726 Published · ppublish English Journal Article

Molecular analysis of the Bacillus subtilis recF function.

Molecular & general genetics : MGG ·Vol. 228 ·No. 3 ·1991-09-00 ·Pages 393-400

Alonso JC, Stiege AC

Abstract

recF resides between the dnaN and gyrB genes of Bacillus subtilis. The recF15 mutation results in replacement of a glutamate residue in the wild type with a lysine residue in the mutant RecF protein. We investigated the in vivo regulation of recF using a transcriptional fusion to the xylE gene and assaying mRNA production. We found that novobiocin leads to a four-fold induction in recF gene expression, but this is not observed in a gyrB mutant strain. Enhancement of expression of the recF gene in the presence of novobiocin is unrelated to the SOS response. The RecF protein, which has a predicted molecular mass of 42.2 kDa, does not seem to be involved in its own regulation.

Related Genes
dnaN gyrB recF xylE
MeSH Terms
Bacillus subtilis/genetics DNA Repair Gene Expression/drug effects Genes, Bacterial Novobiocin/pharmacology Plasmids RNA, Bacterial/biosynthesis RNA, Messenger/biosynthesis SOS Response, Genetics Topoisomerase II Inhibitors Transcription, Genetic
Chemicals
RNA, Bacterial RNA, Messenger Topoisomerase II Inhibitors Novobiocin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Alonso J C
Max-Planck-Institut für Molekulare Genetik, Berlin, Federal Republic of Germany.
Stiege A C
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00260632
Published
1991-09-00
Pages
393-400
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00260632
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