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PMID: 171662 Published · ppublish English Journal Article

Receptor-mediated shifts in cGMP and cAMP levels in neuroblastoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 9 ·1975-09-00 ·Pages 3472-6

Matsuzawa H, Nirenberg M

Abstract

3':5'-cGMP levels of neuroblastoma N1E-115 cells increase as much as 200-fold upon activation of muscarinic acetylcholine receptors, resulting in intracellular cGMP concentrations greater than 600 pmol/mg of protein. The cells also have receptors for adenosine which mediate an increase in 3':5'-cAMP levels. Unexpectedly, prostaglandin E1 was found to increase the concentrations of both cGMP and cAMP. Carbamylcholine, adenosine, and PGE1 were added to cells separately and in pairs to determine the effect of one compound on cell responses to another. Reciprocal inhibition, unilateral inhibition, additive, and nonadditive responses were observed with respect to cGMP and cAMP levels when different pairs of receptors were activated simultaneously.

MeSH Terms
Adenosine/pharmacology Atropine/pharmacology Carbachol/pharmacology Cell Line Cyclic AMP/metabolism Cyclic GMP/metabolism Drug Interactions Prostaglandins E/pharmacology Receptors, Cholinergic Receptors, Drug Tubocurarine/pharmacology
Chemicals
Prostaglandins E Receptors, Cholinergic Receptors, Drug Atropine Carbachol Cyclic AMP Cyclic GMP Adenosine Tubocurarine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Matsuzawa H
Nirenberg M
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.9.3472
Published
1975-09-00
Pages
3472-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433016
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.9.3472 PMC Full Text View journal details
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