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PMID: 1054841 Published · ppublish English Journal Article

Morphine receptors as regulators of adenylate cyclase activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 2 ·1975-02-00 ·Pages 590-4

Sharma SK, Nirenberg M, Klee WA

Abstract

Morphine inhibits adenylate cyclase (EC 4.6.1.1) activity of neuroblastoma times glioma hybrid cells. The inhibition is stereospecific and is reversed by the antagonist, naloxone. The relative affinities of narcotics for the opiate receptor agree well with their effectiveness as inhibitors of adenylate cyclase. Morphine-sensitive and -insensitive cell lines were found, and the degree of sensitivity was shown to be dependent upon the abundance of narcotic receptors. Thus, morphine receptors are functionally coupled to adenylate cyclase. A molecular mechanism for narcotic addiction and tolerance is proposed.

MeSH Terms
Adenylyl Cyclase Inhibitors Animals Cell Line Glioma Hybrid Cells Mice Morphine/antagonists & inhibitors,metabolism,pharmacology Naloxone/pharmacology Neuroblastoma Prostaglandins/pharmacology Rats Receptors, Drug
Chemicals
Adenylyl Cyclase Inhibitors Prostaglandins Receptors, Drug Naloxone Morphine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sharma S K
Nirenberg M
Klee W A
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.2.590
Published
1975-02-00
Pages
590-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432359
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.2.590 PMC Full Text View journal details
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